C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2025
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2024
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2023
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2022
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2021
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2020
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2019
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of Sciencespring 2018
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Thu 12:00–13:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2017
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 20. 2. to Mon 22. 5. Thu 12:00–13:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2016
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 13:00–14:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceSpring 2015
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 17:00–18:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2013
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 16:00–17:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Mon 15:00–16:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Thu 12:00–13:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 16:00–17:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2009
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 17:00–18:50 C05/114
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Thu 15:00–16:50 C05/107
- Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods
- seminar - calculations and other training related to applied enzymology
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2007
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2005
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2004
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2003
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2002
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2001
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2000
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2024
The course is not taught in Autumn 2024
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2023
The course is not taught in Autumn 2023
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2022
The course is not taught in Autumn 2022
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of Scienceautumn 2021
The course is not taught in autumn 2021
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2020
The course is not taught in Autumn 2020
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2019
The course is not taught in Autumn 2019
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2018
The course is not taught in Autumn 2018
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of Scienceautumn 2017
The course is not taught in autumn 2017
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Learning outcomes
- The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2016
The course is not taught in Autumn 2016
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2015
The course is not taught in Autumn 2015
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2014
The course is not taught in Autumn 2014
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminar
- Assessment methods
- Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Applied enzymology - seminars
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Applied enzymology )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. At its end students should understand this topics - Bioanalytical applications of enzymes. Technics of enzyme immobilization. Enzyme biosensors and enzyme reactors. Enzymes in immunochemical techniques. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Teaching methods
- seminars
- Assessment methods
- credit
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
C5991 Aplikovaná enzymologie - cvičení
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- NOW( C5990 Aplikovaná enzymologie )
basic overview of biochemistry - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Applied Biochemistry (programme PřF, B-CH)
- Course objectives
- Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
- Syllabus
- 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
- Assessment methods (in Czech)
- seminář - řešení praktických úloh z oblasti aplikované enzymologie
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Spring 2025, recent)