PřF:C6200 Biochemical Methods - Course Information
C6200 Biochemical MethodsFaculty of Science
- Extent and Intensity
- 4/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- Tue 12:00–13:50 B11/205, Wed 13:00–14:50 B11/205
- ( c3181 Biochemistry I && C4182 Biochemistry II )|| C3580 Biochemistry
Basic knowledge of biochemistry, analytical and physical chemistry.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 11 fields of study the course is directly associated with, display
- Course objectives
- The students should understand at the end of the course the basic methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of enzymes and biopolymers, determination of purity and molecular properties of enzymes, spectroscopic, electrochemical and physical methods of analysis in biochemistry.
- Learning outcomes
- At the end of this course the students should not only theoretically understand but be able practically apply the methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of proteins, nucleic acids and other biopolymers, methods for determination their purity and molecular properties, spectroscopic, electrochemical and physical methods of analysis in biochemistry. They should be able to apply these knowledges in their scientific research working on bachelor, master and PhD theses and finally during their following employment as well.
- 1. Introduction, the strategy of treatement of biological material 2. Disintegration, centrifugation, sedimentation analysis 3. Phase separation, precipitation, extraction. membrane separation, Concentration and lyophilisation, water treatment 4. Chromatographic methods, principles (adsorption, ion-exchange), chromatofocusation 5. Reversed phase chromatography, ion pair chromatography, hydrophobic, GPC, affinity, gaz chromatography 6. Electromigration methods, principles, zone electrophoresis 7. IEF, isotachophoresis, blotting 8. Methods of determination of mol. mass and shape of molecules, mass spectrometry, Osmometry, Viscosimetry, translation and rotation diffusion 9. X-ray analysis, electrochemical methods, principles, potentiometry, ISE, biosensors 10. Amperometry, polaropgraphy, voltammetry, calorimetry, biochemical applications. Fast reactions, ion exchange, ligand-polymer interactions, methods of determination binding parameters. 11. Spectrometry. Electron spectra, transitions, ground and excited state, influence of solvents, UV-VIS spectrometry. Use for the study of protein structure 12. Luminiscence methods. Physical parameters, fluorescence quenching, energy transfer, fluorescence polarisation, phosphorimetry, 13. IR spctroscopy, Raman spectroscopy, ORD, CD 14. NMR, EPR, Mossbauer spectroscopy
- KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987. 430 s. info
- ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986. 199 s. info
- KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980. 349 s. info
- Teaching methods
- Lectures with a demonstration of the taught topic by means of demonstration videos and programs that are available for the students on web page of this course.
- Assessment methods
- Basic lecture given by Prof. Zbořil and Prof. Glatz, with invited external lectures that are expert in specific fields of biochemical methods. The exam is in the oral form. Examiner Prof. Zbořil. The time for the test is one hour, the grades are A to F on the basis of each question classification.
- Language of instruction
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
- Listed among pre-requisites of other courses