PřF:C7235 Fluorescence methods - excer - Course Information
C7235 Fluorescence methods in life sciences - excerciseFaculty of Science
- Extent and Intensity
- 0/2/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
- doc. Mgr. Ctirad Hofr, Ph.D. (seminar tutor)
Mgr. Vratislav Peška, Ph.D. (seminar tutor)
Mgr. Ivona Nečasová, Ph.D. (seminar tutor)
Mgr. Eliška Janoušková, Ph.D. (seminar tutor)
Mgr. Milan Hluchý (seminar tutor)
Mgr. Martina Dvořáčková, Ph.D. (seminar tutor)
Mgr. Pavel Veverka (seminar tutor)
Mgr. Martin Stojaspal (seminar tutor)
Ing. Tomáš Janovič (seminar tutor)
- Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- Prerequisites (in Czech)
- NOW ( C7230 Fluorescence methods )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 7/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- The main objective of the practical training course is to acquire practical experience with modern spectroscopic methods employed in the analysis of protein-protein and protein-DNA interactions. From the methodical approaches, the main emphasis is put on highly sensitive fluorescent methods and their practical use in biological laboratories. The practical one week course, complementing lectures of Bi7230, enables trainees to corroborate their theoretical knowledge about methodical principles using modern instrumentation of research laboratories. Training is focused on the effective development of skills required for application of specialized techniques. These techniques have become indispensable for recent tasks of basic and applied research in today’s interdisciplinary concept of biological sciences.
- Spectral characterization of proteins and nucleic acids
- Accurate determination of concentration of nucleic acids and proteins by fluorescence and absorption spectroscopy (colorimetry)
- Effects of pH and temperature on spectral properties of fluorophores
- Measurement of intrinsic fluorescence of BSA, tryptophan, and thyroxin
- Preparation of fluorescently labeled DNA
- Spectroscopic characterization of fluorescently labeled DNA
- Visualization of macromolecules after electrophoretic separation
- Use of resonance energy transfer (FRET) for detection of DNA hybridization
- Fluorescence quenching after relaxation of loop structure of fluorescently labeled DNA
- Real-time PCR – Detection of DNA amplification
- Fluorescence microscopy (fluorescence in situ hybridization)
- Fluorescence anisotropy – Study of protein binding to a fluorescently labeled DNA
- Lakowicz J.R.: Principles of Fluorescence Spectroscopy. Third Edition, Springer + Business Media, New York, 2006
- Assessment methods
- Language of instruction
- Further Comments
- The course is taught annually.
The course is taught: in blocks.
- Teacher's information