PřF:C7998 Basics of Experimental NMR Spe - Course Information
C7998 Basics of Experimental NMR SpectroscopyFaculty of Science
- Extent and Intensity
- 0/0/1. 1 credit(s). Type of Completion: z (credit).
- Mgr. Lukáš Maier, Ph.D. (seminar tutor)
Mgr. Zdeněk Moravec, Ph.D. (seminar tutor)
prof. RNDr. Radek Marek, Ph.D. (alternate examiner)
- Guaranteed by
- prof. RNDr. Radek Marek, Ph.D.
Department of Chemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Chemistry - Chemistry Section - Faculty of Science
- C1020 General Chemistry
Basic knowledge of NMR spectroscopy.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 18 fields of study the course is directly associated with, display
- Course objectives
- Students will learn basics of 1D NMR measurement. Students will be able to prepare NMR sample, measure NMR spectra and process data of common nuclei : 1H, 13C, 19F, 11B, 31P at the end of the course.
- Learning outcomes
- Student will be able to: - prepare sample for NMR; - select and set NMR experiment; - control measurement during acquisition;
- NMR lab introducing
- AVANCE 300 MHz NMR spectrometer: construction of cryomagnet
- NMR probes
- Temperature unit
- Hardware and software (TopSpin)
- Basics of pulse-field NMR spectrometry:
- deuterium stabilization (lock)
- tunning of field homogenity (shimming)
- acquisition parameters, pulse sequences, FID (Free induction decay)
- matemathical operation with FIDs – windows functions Fourier transformation, data processing, phase corrections
- Measurement and interpretation of 1H NMR spectra
- Measurement of 1H NMR (300 MHz) spectra of unknown compound CDCl3 at 298 and 313 K under operator guidance
- 1H NMR spectra interpretation: o integral intensity, spin-spin couplings, chemical Shift determinativ and overall structural elucidation
- Measurement and interpretation of 13C NMR spectra (APT), 11B and 19F NMR or other nuclei on regest
- Measurement of 13C NMR (75MHz) spectra with and withou proton decoupling under operator guidance
- APT spectra
- Interpretation of 13C and APT NMR spectra
- Measurement and interpretation of 11B and 19F NMR spectra of unknown compound.
- recommended literature
- Claridge, Timothy D.W. High-Resolution NMR Techniques in Organic Chemistry, Amsterdam, Pergamon, 1999, ISBN 0-08-042798-7
- Braun, S. - Kalinowski, H.O. - Berger, S. 100 and More Basic NMR Experiments, Weinheim, VCH, 1996, ISBN 3-527-29091-5
- Teaching methods
- Practical training
- Assessment methods
- Full attandance is mandatory. Student has to performed basic NMR experiments (1H, 13C and APT).
- Language of instruction
- Follow-Up Courses
- Further Comments
- The course is taught each semester.
The course is taught: in blocks.
- Teacher's information