PřF:C8042 Molecular Spectrometry - Course Information
C8042 Molecular SpectrometryFaculty of Science
- Extent and Intensity
- 1/0/0. 2 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- prof. RNDr. Viktor Kanický, DrSc. (lecturer)
- Guaranteed by
- prof. RNDr. Viktor Kanický, DrSc.
Chemistry Section - Faculty of Science
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 9 fields of study the course is directly associated with, display
- Course objectives
- Bouguert-Lamber-Beer law, absorption spectra, instrumentation, errors, calibration, optimization difference spectrometry, derivative and two-wavelength spectrometry, wavelength modulation spectrometry, extraction spectrometry, sensitivity and limits, complexation in solutions, spectrophotometric reagents, thermal lensing and intracavity laser spectrometry.
- 1. Origin of discoloration of compounds, absorption spectra, influence of chemical and electronic structure of compounds and ions, isosbestic point. 2. Bouguert-Lambert-Beer's law, apparent and real deviations from its validity. Influence of monochromatization, light scatter, fluorescence, chemical equilibria, refractive index. 3. Instrumentation, spectrophotometer UV/Vis, radiation sources, dispersion modules, detectors. 4. Errors in spectrophotometry, random errors, systematic errors, instrument errors, errors following from chemical equilibria, calibration of spectrophotometer. 5. Study of spectrophotometric characteristics of inorganic systems in visible region, solvated ions, complexes with chromogenic reagents. 6. Study of complex forming equilibria, method of continual variations, method of ratio of tangents, method of molar ratio, absorbance-pH dependences. 7. Interferences, threshold concentration of interfering constituent, positive and negative error, constant and proportional interference, competition equilibria, overlaps of spectral absorption bands, masking agents. 8. Spectrophotometry and organic analytical reagents, selectivity, sensitivity. 9. Application of spectrophotometry UV/Vis in inorganic elemental analysis, examples of employed methods. 10. Extraction spectrophotometry. 11. Spectrophotometry and kinetic methods, differential technique, method of tangents, method of fixed time, method of fixed concentration of reaction product, integral technique, catalytic reactions, enzyme catalyzed reactions. 12. Multicomponent analysis 13. Photoacoustic spectrometry. 14. Statistical evaluation of results.
- Language of instruction
- Further Comments
- The course is taught annually.
The course is taught: every week.