PřF:C9088 RNAseq analysis - Course Information
C9088 RNAseq analysisFaculty of Science
- Extent and Intensity
- 1/1/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Charlotte Sonenson, Ph.D. (lecturer)
doc. RNDr. Radka Svobodová, Ph.D. (lecturer)
Mgr. Eva Budinská, Ph.D. (lecturer)
- Guaranteed by
- doc. RNDr. Radka Svobodová, Ph.D.
National Centre for Biomolecular Research - Faculty of Science
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- Course Enrolment Limitations
- The course is offered to students of any study field.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20
- Course objectives
- The course is focused on obtaining of practical knowledge in a field of RNAseq analysis.
Basic idea of the subject is to focus on individual steps of RNAseq analysis. In each step, its realization is described, relevant software tools are showed and applied on selected examples.
At the end of the course, the student will be able to:
Work with methods and software tool for RNAseq analysis.
Perform the whole process of RNAseq analysis.
Interpret and discuss outputs of individual steps.
- Learning outcomes
- After finishing of the course, a student will be able to describe methods for realization of individual steps during RNAseq analysis. The student will be also able to use software tools, implementing the methods. Last but not least, the student can describe and perform complete workflow for RNAseq analysis.
- During this course, we will go through the different steps of an RNA-seq experiment, including quality control, alignment of reads to a reference sequence, quantification of the expression of features, differential expression analysis and downstream analysis. We will discuss pitfalls, advantages and disadvantages of different approaches, and the participants will apply the methods.
- Teaching methods
- lectures with practical demonstrations
- Assessment methods
- Written examination
- Language of instruction
- Further Comments
- The course is taught: in blocks.