SumFSci Summer Internships in laboratories of Faculty of Science

Faculty of Science
Spring 2017
Extent and Intensity
0/0/40. 10 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Přemysl Lubal, Ph.D. (seminar tutor)
Mgr. Markéta Machálková, Ph.D. (seminar tutor)
Mgr. Tomáš Vaculovič, Ph.D. (seminar tutor)
Mgr. Vít Vykoukal, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Viktor Kanický, DrSc.
Faculty of Science
Contact Person: Mgr. Petr Bureš
Supplier department: Faculty of Science
Prerequisites
To be a bachelor student at any university in United States
Course Enrolment Limitations
The course is offered to students of any study field.
Syllabus
  • Research stay in laboratories of Faculty of Science, in the period 4. 6. - 4. 7. 2017



    assoc. prof. Premysl Lubal (mentor), Michael Ragone (469801), The University of Arizona, Tucson, AZ
    Topic: The study of thermodynamic and kinetic properties of metal complexes of macrocyclic ligands

    The synthesis and study of coordination properties of macrocyclic ligands having pendant functional groups is stimulated by application of their metal complexes in medicine. Metal complexes with radioisotopes are employed for theranostics= therapy and diagnostics purposes, Gd(III) complexes are used as contrast agents in magnetic resonance imaging as well as other Ln(III) complexes exhibiting luminescence properties for determination of ions important in vivo. These ions must be bound tightly in their metal complexes of high thermodynamic stability and kinetic inertness since the metal ions could not be released from their metal complexes due to their toxicity. The macrocyclic-unit moiety for coordination of metal ions are usually attached via linker to targeting molecule responsible for their distribution in vivo in body. The goal of this project is research of metal complexes from both thermodynamic and kinetic point of view in order to optimize the structural design of new macrocyclic ligands suitable for possible biomedical applications. The candidate – student in bachelor study program should have basic knowledge of coordination, physical and analytical chemistry in order to carry out the research in this field.



    Mgr. Vít Vykoukal (mentor), Emily Chang (469800), Georgia Institute of Technology, Atlanta
    Topic: New routes for nanoalloy preparation

    The combination of inorganic and materials chemistry enables the use of new precursors and their application in the synthesis of new materials. Synthesis of metal nanoalloys is nowadays a very attractive and progressive topic, which is intensely studied. The preparation of nanoalloys is a challenging task due to their chemical, phase, and morphological variability. Bimetallic nanoalloys exhibit different properties from their macroscopic (bulk) equivalent, such as plasmon resonance,1 spinodal decomposition,2 depression of melting point,3,4 different miscibility5 and higher catalytic activity.6–8 The resulting size, shape and size distribution of nanoalloys are determined by the nature of the precursors, and this is a motivation for the preparation and study of new molecular precursors.
    The participant will have the opportunity to learn the basics of metal complex synthesis, thermolytic and reductive preparation of nanoalloy materials, as well as techniques required for characterization of nanoparticles. Basic laboratory experience is advisable.



    Mgr. Markéta Machálková (mentor), Everett Montalvo (469802), Kansas State University, Kansas
    Topic: MALDI MS imaging of 3D cell cultures

    Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an analytical method allowing visualization of molecular species such as lipids, proteins, drugs or metabolites across the tissues and organisms. The basic principle is spot to spot laser desorption of the sample surface and separation and detection of the molecules based on their mass to charge ratio. In our experiments, the sample preparation starts with the cultivation of 3D cell lines, their isolation and cryo-sectioning. Thin slices are then covered by matrix (small organic acid) either by sublimation or spraying. Additional steps as tissue washing or trypsin digestion may be employed. Samples are further analyzed and imaged by MALDI TOF mass spectrometer. The student will learn how to prepare samples for MALDI MS imaging analyses from the very beginning and will be able to analyze those by mass spectrometer and evaluate the data. The experiments may be focused either on lipid, protein or cancerostatic analysis, based on the actual demand.



    Tomáš Vaculović, Ph.D. (mentor), Emma Hansen (469799), Kansas State University, Kansas
    Topic: Imaging of elements in biological tissues

    Laser ablation connected with inductively coupled plasma and mass spectrometry (LA-ICP-MS) offers the ability of imaging of elements in the different types of biological tissues with very good spatial resolution from units to hundreds µm. This method is used in biomedical research such as is study of distribution of elements during various types of cancer diseases. A wide range of application also provides information about distribution of essential elements in organs (liver, kidneys). Aim of this study will be to determine the distribution of elements of interests in tumour tissues (e.g. prostatic tumour) and organ (kidney and liver) tissues. The participant will have the opportunity to learn and understand this analytical technique with very good spatial resolution and detection limit as well as sample preparation and data evaluation.
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught only once.
The course is taught: in blocks.

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