PřF:CG010 Proteomics - Course Information

CG010 Proteomics

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
doc. Mgr. Jan Havliš, Dr. (lecturer)
doc. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. Mgr. Jan Paleček, Dr. rer. nat. (lecturer)
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research - Faculty of Science
Supplier department: National Centre for Biomolecular Research - Faculty of Science
Mon 18. 9. to Fri 15. 12. Tue 9:00–10:50 A2-211
basics in biochemistry, analytical and physical chemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
major goal of this subject is to acquaint students overall and generally with proteomics as a field, with its content and main aspects.
Learning outcomes
at the end of the course students should be able:
to understand and to explain terms related to proteomics (protein biosynthesis, sequence, structure and interactions);
to know relations between protein properties and methods of their study
to use this knowledge on proteins and their complexes to follow their role in organisms.
  • Jan Havliš
  • Introduction into proteomics – definition of proteome and proteomics. Why it is so important to study proteome and its changes: postgenomic era and what to do with information, which we cannot read, genotype vs. phenotype, what does happen during expression, from gene to protein and back. Approaches of contemporary proteomics: Expression, purification and analysis of recombinant proteins, analysis of relations between structure and function, differential proteomics, analysis of post-translational modifications
  • Proteins - biosynthesis of proteins, basic structure hierarchy, basic protein properties (size and shape, polarity, charge, reactivity). Expression proteomics - the conditions and regulation of gene products expression (under different conditions, also ontogenically), protein identity: protoprotein, protein splicing (intein, extein), post-translational modifications, transport, localisation of proteins (eg. cell membrane proteins, secretome), degradation, relations between genotype and phenotype.
  • Tomáš Klumpler
  • structural proteomics - domains, tertiary structure, protein folding, structure and environment relations, allostery, structure modelling
  • Jan Paleček
  • Protein interactions - domains, surface - bond types - interactome (maps, evolution) - protein complexes - simple (dimers), multicomponential (SMC...), molecular machinery.
  • David Potěšil
  • Bioinformatics and proteins - Mw, pI, primary structure, PTMs, domains, tertiary structure, gene ontology (GO), phylogenetic similarity (mutations, proteins from organisms with unsequenced genomes), protein complexes.
  • Zbyněk Zdráhal
  • Importance and utilisation of proteomics - role of proteomics in basic research, application of proteomics (disease diagnostics, pathogene identification)
    recommended literature
  • TWYMAN, R.M. Principles of proteomics. : BIOS Scientific Publishers, 2008. ISBN 1 85996 273 4. info
  • Proteomics. Edited by Timothy Palzkill. New York: Kluwer Academic Publishers, 2002. viii, 127. ISBN 0-306-46895-6. info
Teaching methods
The lecture is based on ppt presentations and their explication. Presentations themselves will be available as study materials (black-and-white printable pdf with high resolution and restricted access rights). It is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks in Czech language covering certain parts of the subject. Students will test their acquired knowledge by means of "in-rough" tests, which will have similar form and content as the final examination test, they will be corrected to give the feed-back, but not graded.
Assessment methods
written & oral examination; single-choice test consists of sixty questions with five choices per question. to pass the test, it is necessary to answer correctly at least 60 % of the questions; test covers complete content of the course. the examination will be finished by oral examination.
Language of instruction
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018.
  • Enrolment Statistics (autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2017/CG010