PřF:S2006 Fluorescence in life sciences - Course Information
S2006 Fluorescence methods in life sciences - a journey from molecules to cellsFaculty of Science
- Extent and Intensity
- 2/0. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
- doc. Mgr. Ctirad Hofr, Ph.D.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- Tue 13:00–14:50 A2-211
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Definition and characteristics of absorption, fluorescence and phosphorescence Instrumentation - spectrophotometer, spectrofluorometer Steady-state fluorescence Time-resolved fluorescence Fluorescence anisotropy and its change after interaction of macromolecules Absorption spectroscopy in biological analysis of molecules Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules Intrinsic fluorescence of biologically important molecules Extrinsic fluorescence – fluorescent labels, probes, and indicators Fluorescence labeling of DNA and proteins Fluorescence microscopy Analytical applications of fluorescence for concentration determination Examples of fluorescence application in biology
- LAKOWICZ, Joseph R. Principles of fluorescence spectroscopy. 3rd ed. New York: Springer, 2006. xxvi, 954. ISBN 0387312781. info
- Language of instruction
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
- Listed among pre-requisites of other courses
- Teacher's information