Bi8120 Applied Cell Biology

Faculty of Science
Spring 2024
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
doc. RNDr. Jakub Neradil, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Mon 10:00–11:50 B11/333
Prerequisites
Bi1700 Cell Biology && ( Bi4010 Essential molecular biology || Bi4020 Molecular biology )
Basics of cell and molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
The aim of this course is to extend the basics of animal cell biology to the practical applications, especially in reproductive medicine, biological therapy for cancer, or regenerative medicine. Certain methodological approaches used in research as well as in practice will be discussed in detail.
Learning outcomes
At the end of the course, the students should be able:
- to understand in detail the specific aspects of applied animal cell biology (including human cells);
- to explain the principles of main methodological approaches to the animal cells;
- to interpret the practical applications of this knowledge in selected areas of medicine.
Syllabus
  • 1) STUDY OF ANIMAL CELLS UNDER IN VITRO CONDITIONS: development of cell cultivation in vitro, culture conditions for mammalian and human cells in vitro, types of cell cultures, biological features of normal and transformed cell lines, practical applications, archiving and cell culture collections;
  • 2) IN VITRO METHODS FOR STUDYING OF CELL LIFE AND DEATH: types of cell death, apoptosis, methodological approaches for apoptosis studies, cytotoxicity vs. viability, medical aspects of apoptosis;
  • 3) FERTILIZATION A EMBRYOGENESIS: meiosis and gametogenesis, mechanisms of fertilization, early embryogenesis, hybrids and chimeras;
  • 4) ASSISTED REPRODUCTION OF ANIMALS AND CLONING: assisted reproduction of animals - overview of methods and practical applications, history and methods of animal cloning - parthenogenesis, splitting techniques, cloning using chimera production, SCNT method, cybrid production;
  • 5) MEDICALLY ASSISTED REPRODUCTION: infertility and its causes, history of assisted reproduction in medicine, overview of established methods, in vitro fertilization, human cloning, production of artificial gametes;
  • 6) CELL DIFFERENTIATION: differentiation potential of animal cells, differentiation pathways, growth factors, induced differentiation;
  • 7) EMBRYONIC AND ADULT STEM CELLS: biological features of stem cells, adult stem cells, embryonic stem cells, stem cell lines under in vitro conditions, induced pluripotent stem cells;
  • 8) CANCER STEM CELLS: hypotheses of cancer stem cell origin, biological features, markers of cancer stem cells and functional assays on the phenotype, possibilities of targeted anticancer therapy;
  • 9) CELL THERAPIES: biological treatment of cancer, gene therapies, cell therapies, the usage of adult and embryonic stem cells;
  • 10) TISSUE AND ORGAN REPLACEMENTS: strategies for production of tissue and organ replacements under in vitro conditions, examples of medical applications in practice, 3D-bioprinting, production of xenobots.
Literature
    recommended literature
  • Principles of tissue engineering. Edited by R. P. Lanza - Robert S. Langer. 4th ed. Amsterdam: Academic Press. xlviii, 18. ISBN 9780123983589. 2014. info
  • Handbook of stem cells. Edited by R. P. Lanza - Anthony Atala. 2nd ed. Amsterdam: Elsevier. s. i-xviii. ISBN 9780124017184. 2013. info
  • Essentials of stem cell biology. Edited by R. P. Lanza. 2nd ed. Amsterdam: Elsevier/Academic Press. xxix, 650. ISBN 9780123747297. 2009. info
  • FRESHNEY, R. Ian. Culture of animal cells : a manual of basic technique. 5th ed. Hoboken, N.J.: Wiley-Liss. xxvi, 642. ISBN 0471453293. 2005. info
Teaching methods
Lectures.
Assessment methods
Oral exam.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023.
  • Enrolment Statistics (recent)
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