S5018 Methods of Protein Characterization

Faculty of Science
Spring 2023
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Recommended Type of Completion: k (colloquium). Other types of completion: zk (examination).
doc. RNDr. Radovan Fiala, CSc. (lecturer)
Mgr. Josef Houser, Ph.D. (lecturer)
Mgr. Pavel Kadeřávek, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. Mgr. Karel Kubíček, PhD. (lecturer)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Guaranteed by
Mgr. Jiří Nováček, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: RNDr. Michaela Fajkusová
Supplier department: Central European Institute of Technology
Basic knowledge of biochemistry and molecular biology.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The aim of this course is to introduce to students present possibilities of methods of Protein Characterization.
  • I. Protein characterization by biochemical methods
  • 1.Biomolecular sample, quality analysis, sample requirements
  • 2.Methods for analysis of biomolecules, complementarity of techniques
  • 3.Biomolecular interactions – methods, comparison, applications
  • 4.Optimization of experiment, global analysis approach
  • 5.High-throughput approach in biomolecular interactions
  • II. Protein characterization by NMR
  • 1. Principles of NMR Spectroscopy
  • 2. What Can NMR Tell about Proteins
  • 3. Protein Structure Determination by NMR
  • 4. Protein Dynamics, Interaction, and Transient States Studied by NMR
  • III. Protein characterization by cryoEM
  • 1. Introduction to electron microscopy - Applications of electron microscopy in the life-science research
  • 2 Electron microscopy imaging - Transmission electron microscope, cryo-electron microscopy, principles of image formation.
  • 3. Data analysis - Alignment of the data in 2D, methods for 3D structure determination
  • IV. Protein characterization by single crystal X-ray diffraction and Bio-SAXS
  • 1A. Introduction to single crystal X-ray diffraction
  • 2A. Macromolecular crystallization techniques
  • 3A. Diffraction experiment
  • 4A. The Phase Problem & methods of its solving
  • 5A. Structural model & its refinement
  • 1B. Introduction to Biological Small Angle X-ray Scattering
  • 2B. Basic principles
  • 3B. SAXS experiment
  • 4B. Data analysis
  • 5B. SAXS based modeling
Teaching methods
  • online lectures
    uploaded gradually during the semester in the Interactive syllabus of the course
    students go through the commented presentations and videos individually
  • individual consultations with the lecturers
  • Assessment methods
    Essay on the topic of using a method for protein characterization in my thesis/project/research.
    Language of instruction
    Teacher's information
    Another recommended course: S5017 Mass Spectrometry in Proteomics https://is.muni.cz/auth/predmet/sci/jaro2022/S5017 More information + recommended literature in the Interactive Syllabus of the course.
    The course is also listed under the following terms Spring 2022.
    • Enrolment Statistics (recent)
    • Permalink: https://is.muni.cz/course/sci/spring2023/S5018