Bi2060c Basic microbiology - practice

Faculty of Science
Spring 2014
Extent and Intensity
0/1. 1 credit(s). Type of Completion: z (credit).
Mgr. Jana Čmielová, Ph.D. (seminar tutor)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor)
Guaranteed by
doc. Ing. Martin Krsek, CSc., MSc
Department of Experimental Biology - Biology Section - Faculty of Science
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable of Seminar Groups
Bi2060c/01: Thu 8:00–8:50 E25/111
Bi2060c/02: Thu 9:00–9:50 E25/111
Prerequisites (in Czech)
NOW ( Bi2060 Basic microbiology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to work aseptically, to inoculate and cultivate bacteria, to prepare stained and native microscopic slides, to determine number of bacteria in different samples, to isolate bacterial group from chosen samples, to work with yeasts, to use some basic identification methods.
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Determination of the concentration of antibiotics by microbiological methods. 7. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 12. Reports, test and microscopic examination/determination of unknown bacterial strain.
  • JANDOVÁ, Božena a Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996. 67 s. ISBN 80-210-1374-5.
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical classes, written reports, written test, credit
Language of instruction
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021.
  • Enrolment Statistics (Spring 2014, recent)
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