PřF:Bi7722 Meth.Microorg.Anal.II - Course Information
Bi7722 Methods of Microorganisms Analysis IIFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Tue 15:00–16:50 E25/339
- The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
- Technology of recombinant DNA and their importance for study of microorganisms.
- Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
- Labelling and detection of probes.
- Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
- Microarrays (microchips), their construction and use in microbiology.
- Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
- PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
- Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
- Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
- Different methods of sequencing of nucleic acids and analysis of DNA sequences.
- Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
- Use of microorganisms in search for biologically aktive compounds.
- Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
- required literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005. 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- recommended literature
- Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011. xvi, 936. ISBN 9781555814977. info
- BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007. xviii, 389. ISBN 9788024417196. info
- Teaching methods
- The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
- Assessment methods
- The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
- Language of instruction
- Further Comments
- Study Materials
The course is taught annually.