Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Tue 15:00–16:50 E25/339
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011. xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007. xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2015, recent)
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