SEDLÁČEK, Ivo, Eva KREJČÍ, Anna ANDĚLOVÁ, Michaela SEDLÁČKOVÁ, Iva PORAZILOVÁ and Pavla HOLOCHOVÁ. Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic. Annals of Agricultural and Environmental Medicine. 2012, vol. 19, No 3, p. 409-413. ISSN 1232-1966.
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Basic information
Original name Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic
Authors SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Eva KREJČÍ (203 Czech Republic), Anna ANDĚLOVÁ (203 Czech Republic), Michaela SEDLÁČKOVÁ (203 Czech Republic), Iva PORAZILOVÁ (203 Czech Republic) and Pavla HOLOCHOVÁ (203 Czech Republic).
Edition Annals of Agricultural and Environmental Medicine, 2012, 1232-1966.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Poland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.060
RIV identification code RIV/00216224:14310/12:00061179
Organization unit Faculty of Science
UT WoS 000309327800013
Keywords in English Aeromonas; identification; ribotyping; fatty acids; cpn60 sequencing
Tags AKR, rivok
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 9/4/2013 10:08.
Abstract
Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.
Links
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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