Other formats:
BibTeX
LaTeX
RIS
@inproceedings{1068099, author = {Šobrová, Pavlína and Ryvolová, Markéta and Adam, Vojtěch and Trnková, Libuše and Hubálek, Jaromír and Kizek, René}, address = {Bratislava}, booktitle = {The 1st International Conference on Nanomaterials: Fundamentals and Applications, Book of Abstracts}, keywords = {prion protein;Creutzfeld-Jakob disease;quantum dots;fluorescence;biosensor}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Bratislava}, isbn = {978-80-7097-970-9}, pages = {97-97}, publisher = {Slovak Chemical Society}, title = {Electrochemical sensing of prion proteins using quantum dots}, year = {2012} }
TY - JOUR ID - 1068099 AU - Šobrová, Pavlína - Ryvolová, Markéta - Adam, Vojtěch - Trnková, Libuše - Hubálek, Jaromír - Kizek, René PY - 2012 TI - Electrochemical sensing of prion proteins using quantum dots PB - Slovak Chemical Society CY - Bratislava SN - 9788070979709 KW - prion protein;Creutzfeld-Jakob disease;quantum dots;fluorescence;biosensor N2 - The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. Sensitive and specific detection of abnormal prion protein in blood could provide a diagnostic test or screening assay for animal and human prion diseases. Therefore, diagnostic tests of prion diseases present a unique challenge requiring development of novel assays exploiting properties uniquely possessed by this misfolded protein complex. The characterization and analysis of biomolecules and biological systems in the context of intact organisms is known as in vivo research. A new and exciting direction of research for quantum dots (QDs) is their application as a contrast agent for in vivo imaging. Excellent optical properties of QDs (namely high quantum yield, broad absorption spectra and narrow, symmetric fluorescence spectra from UV to NIR, large effective excitation and emission Stokes shifts), long life-time (high resistance to photobleaching) compared to ordinary fluorophores and stability (resistance to photo- and chemical degradation) predestinate them in usage for imaging and as optical probes for detection of peptides, proteins, nucleic acids and other biomolecules [1-4]. One of our main aims is to find an easy way how to study QDs. Primarily, we optimized microwave synthesis of CdTe QDs, which was coated with mercapto-propionic acid. These particles were then characterized and their electrochemical behaviour was studied using differential pulse voltammetry at hanging mercury drop electrode. Based on our results, it can be concluded that QDs gave several signals related to various chemicals, which was used to their synthesis, and one (-0.75 vs. Ag/AgCl/3 M NaCl) related to QDs itself. Based on the changes of this signal, we can easily quantify the dots and estimate their stability. Moreover, we studied the interactions of QDs with prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 microl. Results showed that the electrochemical signals of prion protein were strongly quenched by CdTe QDs. This phenomenon can be considered as a first step of suggesting of a biosensor for determination of prions in real samples without a sample pre-treatment. ER -
ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Jaromír HUBÁLEK and René KIZEK. Electrochemical sensing of prion proteins using quantum dots. In \textit{The 1st International Conference on Nanomaterials: Fundamentals and Applications, Book of Abstracts}. Bratislava: Slovak Chemical Society, 2012, p.~97. ISBN~978-80-7097-970-9.
|