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@proceedings{1069034, author = {Masařík, Michal and Sochor, Jiří and RuttkayandNedecky, Branislav and Křížková, Soňa and Stiborová, Marie and Eckschlager, Tomáš and Adam, Vojtěch and Kizek, René}, booktitle = {15th International Symposium on Molecular Medicine}, keywords = {oxidative stress; carboplatin; cisplatin; oxaliplatin; doxorubicin; ellipticine; FRAP; DPPH; ABTS; tumour cell lines}, language = {eng}, title = {A determination of oxidative stress in tumour cells treated with cytostatics}, year = {2012} }
TY - CONF ID - 1069034 AU - Masařík, Michal - Sochor, Jiří - Ruttkay-Nedecky, Branislav - Křížková, Soňa - Stiborová, Marie - Eckschlager, Tomáš - Adam, Vojtěch - Kizek, René PY - 2012 TI - A determination of oxidative stress in tumour cells treated with cytostatics KW - oxidative stress KW - carboplatin KW - cisplatin KW - oxaliplatin KW - doxorubicin KW - ellipticine KW - FRAP KW - DPPH KW - ABTS KW - tumour cell lines N2 - Oxidative stress as a response to anticancer drugs is not sufficiently investigated and direct measurement of reactive oxygen species and oxidative stress markers in clinical medicine is still difficult. In our study, oxidative status was studied in model tumour cell lines derived from tumours of the prostate (PC-3, PNT1A, 22RV1). Cell lines were cultured with anticancer drugs carboplatin, cisplatin, oxaliplatin at concentrations of 0, 10, 30 , 50, 80, 100, 150, 180, 200 and 250 micromol/l and drugs as doxorubicin and ellipticine at concentrations of 0, 0.5, 1, 1.5, 2, 4, 6, 10, 12, 15 micromol/l. The rate of oxidative stress was determined by antioxidant activity (FRAP, DPPH and ABTS). Based on the results obtained from oxidative changes in the cells determined by FRAP and ABTS, it was found the lowest antioxidant activity as 0.06 mg of Trolox/l was in cell lines 22RV1 treated with carboplatin at a concentration of 100 micromol/l. PNT1A cells gave the lowest antioxidant activity of 0.35 mg of Trolox/l after exposure to cisplatin concentration of 100 micromol/l. ER -
MASAŘÍK, Michal, Jiří SOCHOR, Branislav RUTTKAY-NEDECKY, Soňa KŘÍŽKOVÁ, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Vojtěch ADAM and René KIZEK. A determination of oxidative stress in tumour cells treated with cytostatics. In \textit{15th International Symposium on Molecular Medicine}. 2012. ISSN~1107-3756.
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