Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines

MASAŘÍK, Michal, Markéta SZTALMACHOVÁ, Martina RAUDENSKÁ, Jaromír GUMULEC, Monika HOLUBOVÁ, Jan BALVAN, Marián HLAVNA, Veronika TANHÄUSEROVÁ, Petr BABULA, Soňa KŘÍŽKOVÁ, Vojtěch ADAM and René KIZEK. Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines. In 17th World Congress on Advances in Oncology. 2012. ISSN 1107-3756.

Basic information

• Original name: Expression of Bcl-2 and other regulatory genes in tumour prostate cell lines
• Authors: MASAŘÍK, Michal, Markéta SZTALMACHOVÁ, Martina RAUDENSKÁ, Jaromír GUMULEC, Monika HOLUBOVÁ, Jan BALVAN, Marián HLAVNA, Veronika TANHÄUSEROVÁ, Petr BABULA, Soňa KŘÍŽKOVÁ, Vojtěch ADAM and René KIZEK.
• Edition: 17th World Congress on Advances in Oncology, 2012.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Greece
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.957
• Organization unit: Faculty of Medicine
• ISSN: 1107-3756
• UT WoS: 000310651600171
• Keywords in English: Bcl-2; p53; c-Fos; c-Jun; Ki-67; prostate cancer; cell lines; qRT-PCR

Abstract

Prostate cancer is the second most frequently diagnosed cancer. Due to its high incidence and mortality, early diagnosis, identification of highly aggressive forms of clinically silent and understanding of disease pathogenesis with typical metabolic differences in order to develop specifically targeted therapy are needed. There have been found several compounds connected with tumourigenesis in prostate cells including Bcl-2. Bcl-2 acts as an inhibitor of apoptosis. It has been suggested that overexpression of Bcl-2 in human cancer cells contributes to their resistance to chemotherapy- and radiotherapy-induced apoptosis and is connected with unfavourable prognosis. In the fact, the majority of human prostate tumours overexpress Bcl-2. Possible associations with other proteins connected with tumour processes as c-Fos, c-Jun, Ki-67, NF-KB and p53 can be assumed. Therefore, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67,NF-KB and p53 genes in two prostate cell lines as 22Rvl cell line, a model of aggressive partially androgen-sensitive prostate cancer and PNTlA cell line, a model of healthy cell line. Moreover, we were interested in the issue how exposure of these cell lines to zinc(Il) ions could influence expression of the above mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation andhigh expression of Bcl-2.