Whole Genome Sequence of Treponema pallidum ssp. pallidum,
Strain Mexico A, Suggests Recombination between Yaws and
Syphilis Strains

J 2012

Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains

PĚTROŠOVÁ, Helena, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lenka MIKALOVÁ, Petra POSPÍŠILOVÁ et. al.

Basic information

Original name

Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains

Authors

PĚTROŠOVÁ, Helena (203 Czech Republic, guarantor, belonging to the institution), Marie ZOBANÍKOVÁ (203 Czech Republic, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Lei CHEN (840 United States of America), Xiang QIN (840 United States of America), Donna M. MUZNY (840 United States of America), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, belonging to the institution)

Edition

PLOS Neglected Tropical Diseases, 2012, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.569

RIV identification code

RIV/00216224:14110/12:00057663

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0001832

UT WoS

000309528100032

Keywords in English

Treponema pallidum; Mexico A strain; genome; syphilis; inter-strain recombination

Abstract

V originále

Treponema pallidum is a Gram-negative spirochete that causes diseases with distinct clinical manifestations and uses different transmission strategies. While syphilis (caused by subspecies pallidum) is a worldwide venereal and congenital disease, yaws (caused by subspecies pertenue) is a tropical disease transmitted by direct skin contact. Currently the genetic basis and evolution of these diseases remain unknown. In this study, we describe a high quality whole genome sequence of T. pallidum ssp. pallidum strain Mexico A, determined using the ?next generation? sequencing technique (Illumina). Although the genome of this strain contains no large rearrangements in comparison with other treponemal genomes, we found two genes which combined sequences from both subspecies pallidum and pertenue. The observed mosaic character of these two genes is likely a result of inter-strain recombination between pallidum and pertenue during simultaneous infection of a single host.

Links

GAP302/12/0574, research and development project

Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Czech Science Foundation

GA310/04/0021, research and development project

Name: Postgenomické studie syfilitické spirochety Treponema pallidum

Investor: Czech Science Foundation, Postgenomic studies on Treponema pallidum, the syphilis spirochete

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NT11159, research and development project

Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR

Citovat

PĚTROŠOVÁ, Helena, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lenka MIKALOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lei CHEN, Xiang QIN, Donna M. MUZNY, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains. PLOS Neglected Tropical Diseases. 2012, vol. 6, No 9, p. "nestrankovano", 10 pp. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0001832.

@article{1069116,
   author = {Pětrošová, Helena and Zobaníková, Marie and Čejková, Darina and Mikalová, Lenka and Pospíšilová, Petra and Strouhal, Michal and Chen, Lei and Qin, Xiang and Muzny, Donna M. and Weinstock, George M. and Šmajs, David},
   article_number = {9},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0001832},
   keywords = {Treponema pallidum; Mexico A strain; genome; syphilis; inter-strain recombination},
   language = {eng},
   issn = {1935-2735},
   journal = {PLOS Neglected Tropical Diseases},
   note = {Art. No. e1832},
   title = {Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains},
   url = {http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001832},
   volume = {6},
   year = {2012}
}

TY  - JOUR
ID  - 1069116
AU  - Pětrošová, Helena - Zobaníková, Marie - Čejková, Darina - Mikalová, Lenka - Pospíšilová, Petra - Strouhal, Michal - Chen, Lei - Qin, Xiang - Muzny, Donna M. - Weinstock, George M. - Šmajs, David
PY  - 2012
TI  - Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains
JF  - PLOS Neglected Tropical Diseases
VL  - 6
IS  - 9
SP  - "nestrankovano"
EP  - "nestrankovano"
SN  - 19352735
N1  - Art. No. e1832
KW  - Treponema pallidum
KW  - Mexico A strain
KW  - genome
KW  - syphilis
KW  - inter-strain recombination
UR  - http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001832
L2  - http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001832
N2  - Treponema pallidum is a Gram-negative spirochete that causes diseases with distinct clinical manifestations and uses different transmission strategies. While syphilis (caused by subspecies pallidum) is a worldwide venereal and congenital disease, yaws (caused by subspecies pertenue) is a tropical disease transmitted by direct skin contact. Currently the genetic basis and evolution of these diseases remain unknown. In this study, we describe a high quality whole genome sequence of T. pallidum ssp. pallidum strain Mexico A, determined using the ?next generation? sequencing technique (Illumina). Although the genome of this strain contains no large rearrangements in comparison with other treponemal genomes, we found two genes which combined sequences from both subspecies pallidum and pertenue. The observed mosaic character of these two genes is likely a result of inter-strain recombination between pallidum and pertenue during simultaneous infection of a single host.
ER  -

PĚTROŠOVÁ, Helena, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lenka MIKALOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lei CHEN, Xiang QIN, Donna M. MUZNY, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequence of Treponema pallidum ssp. pallidum, Strain Mexico A, Suggests Recombination between Yaws and Syphilis Strains. \textit{PLOS Neglected Tropical Diseases}. 2012, vol.~6, No~9, p.~''nestrankovano'', 10 pp. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0001832.

Detailed Information on Publication Record