HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Petr BABULA, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK. ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name ANALYSIS OF MICRO RNAS EXPRESSION IN CELL LINES DERIVED FROM PROSTATE CARCINOMA
Authors HUDCOVÁ, Kristýna, Martina RAUDENSKÁ, Marián HLAVNA, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Petr BABULA, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK.
Edition 12th Workshop of Physical Chemists and Electrochemists, 2012.
Other information
Original language English
Type of outcome Conference abstract
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.729 in 2011
ISBN 978-80-7375-618-5
ISSN 1452-3981
Keywords (in Czech) miRNA; prostate carcinoma; qRT-PCR
Changed by Changed by: doc. MUDr. Jaromír Gumulec, Ph.D., učo 185198. Changed: 29/1/2016 13:35.
Abstract
MiRNAs constitute a large class of single-stranded RNA molecules of 19 to 25 nucleotides that are involved in post-transcriptional gene silencing. MiRNAs not only regulate various developmental and physiologic processes but also are involved in cancer development and can play a role as an important biomarkers. The aim of this study was to determinate the expression levels of selected miRNA in the non-tumor PNT1A line in comparison with tumor lines 22Rv1, PC-3, and LNCaP. We used the TaqMan Gene Expression Assays and quantitative RT-PCR. We observed significantly higher (p<0.01) expression of miRNA 23a in all tumor lines compared to non-tumor PNT1A. Significantly higher (p<0.01) expression of miRNA 224 in 22Rv1 line compared to others and significantly higher (p<0.01) expression of miRNA 375 in all tumor lines in comparison with non-tumor PNT1A.
PrintDisplayed: 5/5/2024 17:37