CERNEI, Natalia Vladimirovna, Markéta KOMINÍKOVÁ, Ondřej ZÍTKA, Jaromír GUMULEC, Michal MASAŘÍK, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Marie STIBOROVÁ, Tomáš ECKSCHLAGER and René KIZEK. HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.
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Basic information
Original name HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION
Authors CERNEI, Natalia Vladimirovna, Markéta KOMINÍKOVÁ, Ondřej ZÍTKA, Jaromír GUMULEC, Michal MASAŘÍK, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Marie STIBOROVÁ, Tomáš ECKSCHLAGER and René KIZEK.
Edition 12th Workshop of Physical Chemists and Electrochemists, 2012.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.729 in 2011
ISBN 978-80-7375-618-5
ISSN 1452-3981
Keywords in English Sarcosine; Hydrophilic interaction chromatography (HILIC); prostate cancer
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 29/10/2012 16:46.
Abstract
Recently, sarcosine has been identified as a potential prostate cancer marker. To provide early determination of this compound, number of methods is still developed. Sarcosine is relatively small molecule, which is polar enough to enable separation by chromatography with reverse phase. For this reason we optimized method for separation by Hydrophilic interaction chromatography (HILIC). The aim of this work was to suggest the method for HILIC separation and determination of sarcosine with electrochemical detection. This aim was reached as shown by the limit of detection (350 nM), which was determined from calibration curve. This method is suitable for determination of sarcosine in real samples such as urine.
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