RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN

a 2012

RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN

SKALÍČKOVÁ, Sylvie, Ondřej ZÍTKA, Jarmila KRUSEOVÁ, Jaromír GUMULEC, Michal MASAŘÍK et. al.

Basic information

Original name

RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN

Authors

SKALÍČKOVÁ, Sylvie, Ondřej ZÍTKA, Jarmila KRUSEOVÁ, Jaromír GUMULEC, Michal MASAŘÍK, Soňa KŘÍŽKOVÁ, Tomáš ECKSCHLAGER, Vojtěch ADAM and René KIZEK

Edition

12th Workshop of Physical Chemists and Electrochemists, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.729 in 2011

ISBN

978-80-7375-618-5

ISSN

Keywords in English

oxidative damage; GSH/GSSG; prostate carcinoma; brain carcinoma; high performance liquid chromatography with electrochemical detection

Změněno: 29/10/2012 16:47, Mgr. Michal Petr

Abstract

V originále

The ratio between oxidized (GSH) and reduced (GSSG) glutathione is considered to be a marker of oxidative damage of an organism. Values of GSH/GSSG below 10 are signal of increased oxidative stress influencing the human organism due to the progressing disease or as a reaction on applied treatment. In our experiment, the GSH/GSSG was determined in patients with brain and prostate carcinoma by high performance liquid chromatography with electrochemical detection. Samples from three groups of patients suffering from brain cancer (Ependymoma anaplastic and medulloblastoma) and prostate cancer were analyzed using optimized method for determination of GSH and GSSG by HPLC-ED. The method recovery of 86-93% was determined and from obtained concentrations the GSH/GSSG ratio was established. The lowest ratio (average of 1.4) was determined in the group (n=4) of patients suffering from Ependymoma anaplastic. Patients (n=15) diagnosed with medulloblastoma the average ratio of 3.5 was determined. Prostate cancer patients exhibit the average ratio of 3.9. In two control groups of healthy persons, the GSH/GSSG of 11.6 and 10.8 was determined. It can be concluded from the results obtained that different tumor diseases have different impact on the redox status of patients.

Citovat

SKALÍČKOVÁ, Sylvie, Ondřej ZÍTKA, Jarmila KRUSEOVÁ, Jaromír GUMULEC, Michal MASAŘÍK, Soňa KŘÍŽKOVÁ, Tomáš ECKSCHLAGER, Vojtěch ADAM and René KIZEK. RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.

@proceedings{1070203,
   author = {Skalíčková, Sylvie and Zítka, Ondřej and Kruseová, Jarmila and Gumulec, Jaromír and Masařík, Michal and Křížková, Soňa and Eckschlager, Tomáš and Adam, Vojtěch and Kizek, René},
   booktitle = {12th Workshop of Physical Chemists and Electrochemists},
   keywords = {oxidative damage; GSH/GSSG; prostate carcinoma; brain carcinoma; high performance liquid chromatography with electrochemical detection},
   language = {eng},
   isbn = {978-80-7375-618-5},
   title = {RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN},
   year = {2012}
}

TY  - CONF
ID  - 1070203
AU  - Skalíčková, Sylvie - Zítka, Ondřej - Kruseová, Jarmila - Gumulec, Jaromír - Masařík, Michal - Křížková, Soňa - Eckschlager, Tomáš - Adam, Vojtěch - Kizek, René
PY  - 2012
TI  - RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN
SN  - 9788073756185
KW  - oxidative damage
KW  - GSH/GSSG
KW  - prostate carcinoma
KW  - brain carcinoma
KW  - high performance liquid chromatography with electrochemical detection
N2  - The ratio between oxidized (GSH) and reduced (GSSG) glutathione is considered to be a marker of oxidative damage of an organism. Values of GSH/GSSG below 10 are signal of increased oxidative stress influencing the human organism due to the progressing disease or as a reaction on applied treatment. In our experiment, the GSH/GSSG was determined in patients with brain and prostate carcinoma by high performance liquid chromatography with electrochemical detection. Samples from three groups of patients suffering from brain cancer (Ependymoma anaplastic and medulloblastoma) and prostate cancer were analyzed using optimized method for determination of GSH and GSSG by HPLC-ED. The method recovery of 86-93% was determined and from obtained concentrations the GSH/GSSG ratio was established. The lowest ratio (average of 1.4) was determined in the group (n=4) of patients suffering from Ependymoma anaplastic. Patients (n=15) diagnosed with medulloblastoma the average ratio of 3.5 was determined. Prostate cancer patients exhibit the average ratio of 3.9. In two control groups of healthy persons, the GSH/GSSG of 11.6 and 10.8 was determined. It can be concluded from the results obtained that different tumor diseases have different impact on the redox status of patients.
ER  -

SKALÍČKOVÁ, Sylvie, Ondřej ZÍTKA, Jarmila KRUSEOVÁ, Jaromír GUMULEC, Michal MASAŘÍK, Soňa KŘÍŽKOVÁ, Tomáš ECKSCHLAGER, Vojtěch ADAM and René KIZEK. RELATION BETWEEN THIOL CONTENT AND TUMORS OF PROSTATE AND BRAIN. In \textit{12th Workshop of Physical Chemists and Electrochemists}. 2012. ISBN~978-80-7375-618-5.

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