ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL
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a 2012

ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES

SZTALMACHOVÁ, Markéta, Marián HLAVNA, Jaromír GUMULEC, Monika HOLUBOVÁ, Petr BABULA et. al.

Basic information

Original name

ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES

Authors

SZTALMACHOVÁ, Markéta, Marián HLAVNA, Jaromír GUMULEC, Monika HOLUBOVÁ, Petr BABULA, Jan BALVAN, Martina RAUDENSKÁ, Veronika TANHÄUSEROVÁ, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK

Edition

12th Workshop of Physical Chemists and Electrochemists, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.729 in 2011

ISBN

978-80-7375-618-5

ISSN

Keywords in English

prostate cell lines; prostate cancer; Bcl-2; c-Fos; c-Jun; Ki-67; NF-kB; p53; qRT-PCR

Změněno: 24/10/2012 11:58, Mgr. Martina Raudenská, Ph.D.

Abstract

V originále

In this study, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines as 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and as PNT1A cell line, a model of healthy cell line. 22Rv1 cells demonstrate different expression patterns in monitored genes compared to PNT1A cells. 22Rv1 cell line has 4.5-fold higher level of Bcl-2 anti-apoptotic gene expression (n = 5). Interestingly, we found no significant differences in c-Jun gene expression in both cell lines. c-Fos gene that together with c-Jun forms important part of AP-1 transcription factor shows 2.5-fold down regulation in 22Rv1 cells. Ki-67, a nuclear protein that is associated with cellular proliferation, is present in 22Rv1 cell line in 2-fold higher concentration compared to PNT1A. Moreover, NF-kB is present in half concentration in 22Rv1cells compared to PNT1A and p53, a key regulator of apoptosis, shows 3.3-fold decreased level compared with PNT1A cell line.

Citovat

SZTALMACHOVÁ, Markéta, Marián HLAVNA, Jaromír GUMULEC, Monika HOLUBOVÁ, Petr BABULA, Jan BALVAN, Martina RAUDENSKÁ, Veronika TANHÄUSEROVÁ, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK. ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.

@proceedings{1070211,
   author = {Sztalmachová, Markéta and Hlavna, Marián and Gumulec, Jaromír and Holubová, Monika and Babula, Petr and Balvan, Jan and Raudenská, Martina and Tanhäuserová, Veronika and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {12th Workshop of Physical Chemists and Electrochemists},
   keywords = {prostate cell lines; prostate cancer; Bcl-2; c-Fos; c-Jun; Ki-67; NF-kB; p53; qRT-PCR},
   language = {eng},
   isbn = {978-80-7375-618-5},
   title = {ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES},
   year = {2012}
}

TY  - CONF
ID  - 1070211
AU  - Sztalmachová, Markéta - Hlavna, Marián - Gumulec, Jaromír - Holubová, Monika - Babula, Petr - Balvan, Jan - Raudenská, Martina - Tanhäuserová, Veronika - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2012
TI  - ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES
SN  - 9788073756185
KW  - prostate cell lines
KW  - prostate cancer
KW  - Bcl-2
KW  - c-Fos
KW  - c-Jun
KW  - Ki-67
KW  - NF-kB
KW  - p53
KW  - qRT-PCR
N2  - In this study, we aimed our attention on determining of expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines as 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and as PNT1A cell line, a model of healthy cell line. 22Rv1 cells demonstrate different expression patterns in monitored genes compared to PNT1A cells. 22Rv1 cell line has 4.5-fold higher level of Bcl-2 anti-apoptotic gene expression (n = 5). Interestingly, we found no significant differences in c-Jun gene expression in both cell lines. c-Fos gene that together with c-Jun forms important part of AP-1 transcription factor shows 2.5-fold down regulation in 22Rv1 cells. Ki-67, a nuclear protein that is associated with cellular proliferation, is present in 22Rv1 cell line in 2-fold higher concentration compared to PNT1A. Moreover, NF-kB is present in half concentration in 22Rv1cells compared to PNT1A and p53, a key regulator of apoptosis, shows 3.3-fold decreased level compared with PNT1A cell line.
ER  -

SZTALMACHOVÁ, Markéta, Marián HLAVNA, Jaromír GUMULEC, Monika HOLUBOVÁ, Petr BABULA, Jan BALVAN, Martina RAUDENSKÁ, Veronika TANHÄUSEROVÁ, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK. ANALYSIS OF SELECTED REGULATORY GENES IN PROSTATE CANCER CELL LINES. In \textit{12th Workshop of Physical Chemists and Electrochemists}. 2012. ISBN~978-80-7375-618-5.

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