Glutation jako marker fotoxidativního stresu u rostlin vystavených různým intenzitám záření. In-vitro studie.

CHMELÍK, Dominik, Miloš BARTÁK, Jaroslava DUBOVÁ and Julie ROTKOVSKÁ. Glutation jako marker fotoxidativního stresu u rostlin vystavených různým intenzitám záření. In-vitro studie. (Glutathione as a marker of photooxidative stress in plants exposed to different intensities of light: In-vitro study.). Online. In Barták, M., Hájek, J., Dubová, J. Rostlinné biotechnologie. Brno: Masarykova univerzita, 2011, p. 17-22. ISBN 978-80-210-5887-3.

Basic information

• Original name: Glutation jako marker fotoxidativního stresu u rostlin vystavených různým intenzitám záření. In-vitro studie.
• Name in Czech: Glutation jako marker fotoxidativního stresu u rostlin vystavených různým intenzitám záření. In-vitro studie.
• Name (in English): Glutathione as a marker of photooxidative stress in plants exposed to different intensities of light: In-vitro study.
• Authors: CHMELÍK, Dominik, Miloš BARTÁK, Jaroslava DUBOVÁ and Julie ROTKOVSKÁ.
• Edition: Brno, Rostlinné biotechnologie, p. 17-22, 6 pp. 2011.
• Publisher: Masarykova univerzita

Other information

• Original language: Czech
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: electronic version available online
• WWW: full_text_available_here
• Organization unit: Faculty of Science
• ISBN: 978-80-210-5887-3
• Keywords (in Czech): glutation; Potinara; in-vitro; bioreaktor RITA
• Keywords in English: glutathione; Potinara; in-vitro; bioreactor RITA
• Tags: Reviewed

Abstract

V práci jsou uvedeny koncentrace glutationu v orideji Potinara hybr. kultivované in-vitro za různé intenzity osvětlení v bioreaktoru RITA.

Abstract (in English)

Micropropagation of plants in vitro is fast and effective way to obtain proliferation of new plant material. However, in vitro cultivated plants may suffer from high light stress when transfered from in vitro conditions. In our study, we focused on orchid plants Potinara hybr. cultivated in vitro and their sensitivity to short-term light stress. The plants were cultivated in temporary immersion system (TIS) RITA. This system enables complete or partial submersion of plants with liquid nutritive medium. In our experiment, plants were submerged 7 times during 24 hours for 5 minutes. Plants were cultivated one month before beginning of the experiment. We investigated the short-term effects of several different light intensities of photosynthetic active radiation (PAR) on the formation of the antioxidative substrate glutathion . Plants were cultivated under low light intensities of PAR (15 micromol. m-2. s-1). During short-term experiment, the plants were exposed to either 800 or 1400 micromol.m-2.s-1 of high light PAR. This exposition lasted for 1 hour. High light-exposed plant material was then subsequently harvested in 20 minute intervals for consequent glutathione analyzes. Concentration of glutathione reduced and oxidized form was measured in plant extract by HPLC. Glutathione thiols were labeled with monobromobimane. When low PAR exposition was used, glutathione concentration showed small increase after 20 minutes, then decrease after 40 minutes and subsequent rapid increase after 60 minutes of the high light exposition. The response of plants to the higher high light PAR (1400 micromol.m-2.s-1) exposition was that the glutathione concentration was much lower after the first 20 minutes and rapid increase of its concentration was measured after 40 and 60 minutes after the high light treatment.

Links

• GA205/09/1876, research and development project: Name: Recentní deglaciace severní části ostrova Jamese Rosse, Antarktida

Investor: Czech Science Foundation, Recent deglaciation of the northern part of James Ross Island, Antarctica