OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM

BYSTRICKÝ, Peter, Markéta KLIŠOVÁ, Marie POKORNÁ and Michaela WIMMEROVÁ. OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM. 2012. ISBN 978-80-86313-34-4.

Basic information

• Original name: OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM
• Authors: BYSTRICKÝ, Peter (703 Slovakia, guarantor, belonging to the institution), Markéta KLIŠOVÁ (203 Czech Republic, belonging to the institution), Marie POKORNÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, belonging to the institution).
• Edition: 2012.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14740/12:00061757
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-86313-34-4
• Keywords (in Czech): NMR ASPERGILLUS FUMIGATUS LEKTIN INTERAKCE ZNAČENÍ SELEKTIVNĚ AMINOKYSELINY
• Keywords in English: NMR ASPERGILLUS FUMIGATUS LECTIN INTERACTIONS AMINO ACID SELECTIVE LABELLING
• Tags: Reviewed

Abstract

A fucose specific Aspergillus fumigatus lectin, AFL1, was subjected to amino acid selective labelling by 15N. The the assignment of key amino acids for the binding can be readily achieved by use of single point mutants. Here we present optimization of recombinant AFL1 lectin over-expression in E.coli cells which is performed in 15N Trp selective supplemented M9 minimal medium. (Semi) optimized parameters were E.coli strain type, concentration of antibiotic, concentration of IPTG for induction, temperature after induction and length of lectin expression after induction.

Links

• SIGA 382, interní kód MU: Name: Interakce protein-sacharid: Podstata rozpoznávaní patogenem (Acronym: LECARB)

Investor: South-Moravian Region, Incoming grants