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@proceedings{1072361, author = {Bystrický, Peter and Klišová, Markéta and Pokorná, Marie and Wimmerová, Michaela}, keywords = {NMR ASPERGILLUS FUMIGATUS LECTIN INTERACTIONS AMINO ACID SELECTIVE LABELLING}, language = {eng}, isbn = {978-80-86313-34-4}, title = {OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM}, year = {2012} }
TY - CONF ID - 1072361 AU - Bystrický, Peter - Klišová, Markéta - Pokorná, Marie - Wimmerová, Michaela PY - 2012 TI - OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM SN - 9788086313344 KW - NMR ASPERGILLUS FUMIGATUS LECTIN INTERACTIONS AMINO ACID SELECTIVE LABELLING N2 - A fucose specific Aspergillus fumigatus lectin, AFL1, was subjected to amino acid selective labelling by 15N. The the assignment of key amino acids for the binding can be readily achieved by use of single point mutants. Here we present optimization of recombinant AFL1 lectin over-expression in E.coli cells which is performed in 15N Trp selective supplemented M9 minimal medium. (Semi) optimized parameters were E.coli strain type, concentration of antibiotic, concentration of IPTG for induction, temperature after induction and length of lectin expression after induction. ER -
BYSTRICKÝ, Peter, Markéta KLIŠOVÁ, Marie POKORNÁ and Michaela WIMMEROVÁ. \textit{OPTIMIZATION OF AFL1 EXPRESSION IN AMINO ACID SELECTIVE MEDIUM}. 2012. ISBN~978-80-86313-34-4.
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