| VOPĚNKOVÁ, Kateřina, Klára MOLLOVÁ, Ivana BUREŠOVÁ a Jaroslav MICHÁLEK. Complex evaluation of human monocyte-derived dendritic cells for cancer immunotherapy. Journal of Cellular and Molecular Medicine. Wiley Blackwell, 2012, roč. 16, č. 11, s. 2827-2837. ISSN 1582-1838. doi:10.1111/j.1582-4934.2012.01614.x. |
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@article{1073177,
author = {Vopěnková, Kateřina and Mollová, Klára and Burešová, Ivana and Michálek, Jaroslav},
article_number = {11},
doi = {http://dx.doi.org/10.1111/j.1582-4934.2012.01614.x},
keywords = {cancer immunotherapy; cytotoxicity; dendritic cell; interleukin-12; maturation; migration},
language = {eng},
issn = {1582-1838},
journal = {Journal of Cellular and Molecular Medicine},
title = {Complex evaluation of human monocyte-derived dendritic cells for cancer immunotherapy},
url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1582-4934.2012.01614.x/abstract},
volume = {16},
year = {2012}
}
TY - JOUR
ID - 1073177
AU - Vopěnková, Kateřina - Mollová, Klára - Burešová, Ivana - Michálek, Jaroslav
PY - 2012
TI - Complex evaluation of human monocyte-derived dendritic cells for cancer immunotherapy
JF - Journal of Cellular and Molecular Medicine
VL - 16
IS - 11
SP - 2827-2837
EP - 2827-2837
PB - Wiley Blackwell
SN - 15821838
KW - cancer immunotherapy
KW - cytotoxicity
KW - dendritic cell
KW - interleukin-12
KW - maturation
KW - migration
UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1582-4934.2012.01614.x/abstract
N2 - Dendritic cell (DC) immunotherapy is capable of generating tumour-specific immune responses. Different maturation strategies were previously tested to obtain DC capable of anti-cancer responses in vitro, usually with limited clinical benefit. Mutual comparison of currently used maturation strategies and subsequent complex evaluation of DC functions and their stimulatory capacity on T cells was performed in this study to optimize the DC vaccination strategy for further clinical application. DC were generated from monocytes using granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4, pulsed with whole tumour cell lysate and then matured with one of five selected maturation strategies or cultured without additional maturation stimulus. DC were characterized with regard to their surface marker expression, cytokine profiles, migratory capacity, allogeneic and autologous T cell stimulatory capacity as well as their specific cytotoxicity against tumour antigens. We were able to demonstrate extensive variability among different maturation strategies currently used in DC immunotherapeutic protocols that may at least partially explain limited clinical benefit of some clinical trials with such DC. We identified DC matured with interferon-gamma and lipopolysaccharide as the most attractive candidate for future clinical trials in cancer immunotherapy.
ER -
VOPĚNKOVÁ, Kateřina, Klára MOLLOVÁ, Ivana BUREŠOVÁ a Jaroslav MICHÁLEK. Complex evaluation of human monocyte-derived dendritic cells for cancer immunotherapy. \textit{Journal of Cellular and Molecular Medicine}. Wiley Blackwell, 2012, roč.~16, č.~11, s.~2827-2837. ISSN~1582-1838. doi:10.1111/j.1582-4934.2012.01614.x.
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