Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products

LANGMAJEROVÁ, Monika, Roman ŘEMÍNEK, Marta ZEISBERGEROVÁ and Zdeněk GLATZ. Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products. In 9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012. 2012. ISBN 978-80-904959-1-3.

Basic information

Original name

Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products

Name in Czech

Aplikace on-line kapilární elektroforézy s hmotnostně spektrometrickou detekcí pro identifikaci produktů činnosti cytochromu P450 2C9

Authors

LANGMAJEROVÁ, Monika (203 Czech Republic, belonging to the institution), Roman ŘEMÍNEK (203 Czech Republic, belonging to the institution), Marta ZEISBERGEROVÁ (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012, 2012

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/12:00057805

Organization unit

Faculty of Science

ISBN

978-80-904959-1-3

UT WoS

000329635000109

Keywords (in Czech)

On-line kapilární elektroforéza; hmotnostní spektrometrie; enzymová reakce

Keywords in English

On-line capillary electrophoresis; mass spectrometry; enzyme reaction

Tags

AKR, CE, CE methods, CYP2C9, diclofenac, drug metabolism, in-capillary reaction

Tags

International impact, Reviewed

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Abstract

V originále

Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Capillary electrophoresis (CE) represents a promising technique in the field of enzyme assays due to miniscule sample consumption and high-throughput by automation. Moreover, CE coupled to mass spectrometry (MS) combines the advantages of both techniques so that CYP metabolism products can be identified on the bases of migration time, molecular weight and fragmentation. A new CE-MS method enabling identification of reaction products provided by clinically important CYP2C9 isoform is thus introduced.

In Czech

Byla vypracována metoda pro on-line identifikaci produktů enzymových reakcí pomocí kapilární elektroforézy s hmotnostní detekcí. Aplikační možnosti metody byly demonstrovány na modelovém systému 4'-hydroxylace diklofenaku činností cytochromu P450 isoformy 2C9.

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Links

GAP206/10/0057, research and development project	Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis
GBP206/12/G014, research and development project	Name: Centrum pokročilých bioanalytických technologií