KUNTOVÁ, Lucie, Roman PANTŮČEK, Jana RÁJOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ, Ivana MAŠLAŇOVÁ and Jiří DOŠKAŘ. Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains. Archives of Microbiology. New York: Springer Verlag, 2012, vol. 194, No 7, p. 607-614. ISSN 0302-8933. doi:10.1007/s00203-012-0797-y.
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Basic information
Original name Characteristics and distribution of plasmids in a clonally diverse set of methicillin-resistant Staphylococcus aureus strains
Name in Czech Charakteristiky a distribuce plazmidů u klonálně rozmanitého souboru meticilin-rezistentních kmenů Staphylococcus aureus
Authors KUNTOVÁ, Lucie (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Jana RÁJOVÁ (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution) and Jiří DOŠKAŘ (203 Czech Republic, guarantor, belonging to the institution).
Edition Archives of Microbiology, New York, Springer Verlag, 2012, 0302-8933.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.905
RIV identification code RIV/00216224:14310/12:00057818
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00203-012-0797-y
UT WoS 000305267200005
Keywords in English Methicillin-Resistant Staphylococcus aureus; Plasmids; Genetic diversity; Antibiotic resistance; Molecular typing
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 10/4/2013 15:56.
Abstract
The aim of this study was to compare the plasmid contents of methicillin-resistant Staphylococcus aureus (MRSA) strains classified into different clonal clusters (CCs). The isolates were collected from 15 Czech hospitals in 2000–2008. Plasmid DNA was detected in 65 (89%) strains and 33 of them harbored more than one plasmid type. Altogether 24 different types of plasmids were identified, ranging in size from 1.3 to 55 kb. Restriction endonuclease analysis, plasmid elimination, DNA hybridization, and sequencing were used for their further characterization. It has been found that the conjugative, erythromycin resistance and enterotoxin D encoding plasmids are harbored by strains from different CCs. On the other hand, chloramphenicol and tetracycline resistance plasmids, and most of the penicillinase and cryptic plasmids were only detected in certain CCs. Especially the pUSA300-like plasmids were found exclusively in the USA300 clone strains. The high diversity in plasmid content detected in the study strains implies that plasmids play a major role in evolution of MRSA clonal lineages.
Links
GA310/09/0459, research and development projectName: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus
Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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