VARGA, Marian, Lucie KUNTOVÁ, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone. FEMS Microbiology Letters. United Kingdom: John Wiley & Sons, Inc., 2012, vol. 332, No 2, p. 146-152. ISSN 0378-1097. doi:10.1111/j.1574-6968.2012.02589.x.
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Basic information
Original name Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
Name in Czech Účinný přenos plazmidů kódujících antibiotické rezistence transdukcí u meticilin-rezistentního klonu Staphylococcus aureus USA300
Authors VARGA, Marian (203 Czech Republic, belonging to the institution), Lucie KUNTOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Vladislava RŮŽIČKOVÁ (203 Czech Republic, belonging to the institution) and Jiří DOŠKAŘ (203 Czech Republic, guarantor, belonging to the institution).
Edition FEMS Microbiology Letters, United Kingdom, John Wiley & Sons, Inc. 2012, 0378-1097.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.049
RIV identification code RIV/00216224:14310/12:00057819
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x
UT WoS 000305334300008
Keywords in English antimicrobial drug resistance; penicillinase plasmids; Staphylococcus bacteriophage; lysogeny; horizontal gene transfer; plasmid transduction
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 16. 11. 2012 17:30.
Abstract
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone.
Abstract (in Czech)
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone.
Links
GA310/09/0459, research and development projectName: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus
Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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