Detailed Information on Publication Record
2012
Different immune response of pigs to Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis infection
ŠTĚPÁNOVÁ, Hana, Barbora PAVLOVÁ, Nikola STROMEROVÁ, Petra ONDRÁČKOVÁ, Karel STEJSKAL et. al.Basic information
Original name
Different immune response of pigs to Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis infection
Name in Czech
Odlišná imunitní odezva prasat na infekce Mycobacterium avium subsp. avium a Mycobacterium avium subsp. hominissuis
Authors
ŠTĚPÁNOVÁ, Hana (203 Czech Republic), Barbora PAVLOVÁ (203 Czech Republic), Nikola STROMEROVÁ (203 Czech Republic), Petra ONDRÁČKOVÁ (203 Czech Republic), Karel STEJSKAL (203 Czech Republic, belonging to the institution), Iva SLANÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Ivo PAVLÍK (203 Czech Republic) and Martin FALDYNA (203 Czech Republic, guarantor)
Edition
Veterinary Microbiology, Amserdam, Elsevier, 2012, 0378-1135
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30102 Immunology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.127
RIV identification code
RIV/00216224:14740/12:00061962
Organization unit
Central European Institute of Technology
UT WoS
000309316000009
Keywords (in Czech)
exprese genů; kauzální faktory; RFLP analýza;
Keywords in English
GENE-EXPRESSION; CAUSAL FACTORS; RFLP ANALYSIS; ANIMALS; STRAINS; TISSUES; HUMANS; CATTLE; PCR
Tags
International impact, Reviewed
Změněno: 10/4/2013 07:47, Olga Křížová
V originále
Mycobacterium avium subsp. avium (MAA) and Mycobacterium avium subsp. hominissuis (MAH) are the most common mycobacterial species isolated from granulomatous lesions in swine in countries with controlled bovine tuberculosis. This study is focused on the immunological aspect of MAA and MAH infection in pigs. We detected induction of humoral and cell-mediated immunity in experimentally infected pigs. Specific antibodies were analyzed in serum by ELISA and the IFN-gamma release assay was used for evaluation of cell-mediated immunity. While MAA induced a significant increase of both types of immune responses, MAH-infected pigs had an unvarying level of specific antibodies and showed low cell-mediated immunity with high individual variability. The subsequent in vitro experiment confirmed the lower immunogenicity of the MAH strain in comparison to MAA. MAH-infected porcine monocyte-derived macrophages showed a weaker induction of pro-inflammatory mediators in comparison to MM, which included mRNA for IL-1 beta, TNF-alpha, IL-23p19, IL-18 and chemokines CCL-3, CCL-5, CXCL-8 and CXCL-10. Additionally, qualitative proteomic analysis revealed 28 proteins exclusively in MAA and 7 proteins unique to MAH. In conclusion, closely related M. avium subspecies MAA and MAH showed different capacities to stimulate the porcine immune system. From a diagnostic point of view, the IFN-gamma release assay showed higher sensitivity than the detection of specific antibodies by ELISA and seems to be an effective tool for discrimination of MAA-infected pigs. In the case of MAH infection, the IFN-gamma release assay could fail because of the low immunogenic capacity of the MAR strain.
In Czech
Mycobacterium avium subsp. avium (MAA) a Mycobacterium avium subsp. hominissuis (MAH) patří k nejčastějším mykobakteriální druhům izolovaným z granulomatózních zánětů prasat. Studie je zaměřena na imunologické aspekty těchto infekcí u prasat. Byla zjištěna aktivace buněčné a hormonální imunity u nakažených prasat. Na základě analýz ELISA, sledování hladiny interferonu gamma a kvantitativní proteomické analýzy byly pozorovány rozdíly v imunitní odezvě vůči MAA a MAH.
Links
| ED1.1.00/02.0068, research and development project |
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| MSM0021622415, plan (intention) |
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| QI101A094, research and development project |
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