KOPECKÁ, Marie. Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls. Microscopy. OXFORD: OXFORD UNIV PRESS, 2013, vol. 62, No 3, p. 327-339. ISSN 0022-0744. doi:10.1093/jmicro/dfs076.
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Basic information
Original name Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls
Authors KOPECKÁ, Marie (203 Czech Republic, guarantor, belonging to the institution).
Edition Microscopy, OXFORD, OXFORD UNIV PRESS, 2013, 0022-0744.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.632
RIV identification code RIV/00216224:14110/13:00067534
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1093/jmicro/dfs076
UT WoS 000320457100001
Keywords in English cell-wall polysaccharides; self-assembly; yeast; fungi; Saccharomyces cerevisiae
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 5. 8. 2013 08:20.
Abstract
Polysaccharides account for more than 90% of the content of fungal cell walls, but the mechanism underlying the formation of the architecture of the cell walls, which consist of microfibrils embedded in an amorphous wall matrix, remains unknown. We used electron microscopy to investigate ten different fungal cell-wall polysaccharides to determine whether they could self-assemble into the fibrillar or amorphous component of fungal cell walls in a test tube without enzymes. The ultrastructures formed by precipitating beta-1,3-glucan and beta-1,6-glucan are different depending on the existence of branching in the molecule. Linear beta-1,3- glucan and linear beta-1,6-glucan precipitate into a fibrillar ultrastructure. Branched beta-1,6-glucan, mannan and glycogen precipitates are amorphous. Branched beta-1,3-glucan forms a fibrillar plus amorphous ultrastructure. Self-assembly among combinations of different linear and branched cellwall polysaccharides results in an ultrastructure that resembles that of a yeast cell wall, which suggests that self-assembly of polysaccharides may participate in the development of the three-dimensional architecture of the yeast cell wall.
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