Redox status expressed as GSH:GSSG ratio as a marker for
oxidative stress in paediatric tumour patients

J 2012

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

ZÍTKA, Ondřej, Sylvie SKALICKOVA, Jaromír GUMULEC, Michal MASAŘÍK, Vojtěch ADAM et. al.

Basic information

Original name

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

Authors

ZÍTKA, Ondřej (203 Czech Republic, guarantor), Sylvie SKALICKOVA (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic), Jaromír HUBÁLEK (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Jarmila KRUSEOVÁ (203 Czech Republic), Tomáš ECKSCHLAGER (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

Oncology Letters, 2012, 1792-1074

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.237

RIV identification code

RIV/00216224:14110/12:00061981

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3892/ol.2012.931

UT WoS

000311021500020

Keywords in English

glutathione; paediatric tumour patients; oxidative stress; high performance liquid chromatography with electrochemical detection

Abstract

V originále

Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, and it is involved in numerous malignancies. Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) may be used as a marker of oxidative stress. The main aim of this study was to determine GSH:GSSG ratio in the blood serum of paediatric cancer patients to use this ratio as a potential marker of oxidative stress. The whole procedure was optimised and the recoveries for both substances were greater than 80% under the optimised conditions. We analysed a group of paediatric patients (n=116) with various types of cancer, including neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract tumour, lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma and retinoblastoma. We simultaneously determined the levels of reduced and oxidised glutathione, and thus, its ratio in the blood serum of the patients. The highest ratio was observed in retinoblastoma patients and the lowest in anaplastic ependymoma. We were able to distinguish between the diagnoses based on the results of the obtained GSH:GSSG ratio.

Citovat

ZÍTKA, Ondřej, Sylvie SKALICKOVA, Jaromír GUMULEC, Michal MASAŘÍK, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Jarmila KRUSEOVÁ, Tomáš ECKSCHLAGER and René KIZEK. Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients. Oncology Letters. 2012, vol. 4, No 6, p. 1247-1253. ISSN 1792-1074. Available from: https://dx.doi.org/10.3892/ol.2012.931.

@article{1074276,
   author = {Zítka, Ondřej and Skalickova, Sylvie and Gumulec, Jaromír and Masařík, Michal and Adam, Vojtěch and Hubálek, Jaromír and Trnková, Libuše and Kruseová, Jarmila and Eckschlager, Tomáš and Kizek, René},
   article_number = {6},
   doi = {http://dx.doi.org/10.3892/ol.2012.931},
   keywords = {glutathione; paediatric tumour patients; oxidative stress; high performance liquid chromatography with electrochemical detection},
   language = {eng},
   issn = {1792-1074},
   journal = {Oncology Letters},
   title = {Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients},
   volume = {4},
   year = {2012}
}

TY  - JOUR
ID  - 1074276
AU  - Zítka, Ondřej - Skalickova, Sylvie - Gumulec, Jaromír - Masařík, Michal - Adam, Vojtěch - Hubálek, Jaromír - Trnková, Libuše - Kruseová, Jarmila - Eckschlager, Tomáš - Kizek, René
PY  - 2012
TI  - Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients
JF  - Oncology Letters
VL  - 4
IS  - 6
SP  - 1247-1253
EP  - 1247-1253
SN  - 17921074
KW  - glutathione
KW  - paediatric tumour patients
KW  - oxidative stress
KW  - high performance liquid chromatography with electrochemical detection
N2  - Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, and it is involved in numerous malignancies. Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) may be used as a marker of oxidative stress. The main aim of this study was to determine GSH:GSSG ratio in the blood serum of paediatric cancer patients to use this ratio as a potential marker of oxidative stress. The whole procedure was optimised and the recoveries for both substances were greater than 80% under the optimised conditions. We analysed a group of paediatric patients (n=116) with various types of cancer, including neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract tumour, lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma and retinoblastoma. We simultaneously determined the levels of reduced and oxidised glutathione, and thus, its ratio in the blood serum of the patients. The highest ratio was observed in retinoblastoma patients and the lowest in anaplastic ependymoma. We were able to distinguish between the diagnoses based on the results of the obtained GSH:GSSG ratio.
ER  -

ZÍTKA, Ondřej, Sylvie SKALICKOVA, Jaromír GUMULEC, Michal MASAŘÍK, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Jarmila KRUSEOVÁ, Tomáš ECKSCHLAGER and René KIZEK. Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients. \textit{Oncology Letters}. 2012, vol.~4, No~6, p.~1247-1253. ISSN~1792-1074. Available from: https://dx.doi.org/10.3892/ol.2012.931.

Detailed Information on Publication Record