SH3b cell wall binding domain of bacteriophage 812 endolysin:
Cloning, expression and structure determination

a 2012

SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK et. al.

Basic information

Original name

SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination

Authors

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA and Roman PANTŮČEK

Edition

Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Belgium

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

Keywords in English

Staphylococcus; bacteriophage; Myoviridae; endolysin; SH3b domain; NMR

Abstract

V originále

Staphylococcus aureus is a major causative agent of human and animal diseases. The increasing number of pathogenic strains resistant to antimicrobial drugs is a serious public health problem that can be solved by applications of phage therapy as a suitable alternative to antibiotics treatment. Currently the research focus on the phage encoded antimicrobial proteins applicable to combat bacterial infections, such as phage endolysins that digest the cell wall of bacteria. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of SPO1-like viruses from family Myoviridae. Preliminary results of the NMR analysis confirmed the presence of the well defined structure of the studied construct, and provided the sequential assignment of all resonance frequencies along the peptide backbone allowing us to assess the presence of the secondary structure motifs.

Links

TA01010405, research and development project

Name: Výzkum stafylokokových bakteriofágových mutant s širokým spektrem hostitelů (Acronym: TAČR/IMUNA-1)

Investor: Technology Agency of the Czech Republic

Citovat

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA and Roman PANTŮČEK. SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination. In Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium. 2012.

@proceedings{1074370,
   author = {Benešík, Martin and Nováček, Jiří and Janda, Lubomír and Dopitová, Radka and Žídek, Lukáš and Doškař, Jiří and Růžičková, Vladislava and Moša, Marek and Pantůček, Roman},
   booktitle = {Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium},
   keywords = {Staphylococcus; bacteriophage; Myoviridae; endolysin; SH3b domain; NMR},
   language = {eng},
   title = {SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination},
   url = {http://events.embo.org/12-virus-microbe/},
   year = {2012}
}

TY  - CONF
ID  - 1074370
AU  - Benešík, Martin - Nováček, Jiří - Janda, Lubomír - Dopitová, Radka - Žídek, Lukáš - Doškař, Jiří - Růžičková, Vladislava - Moša, Marek - Pantůček, Roman
PY  - 2012
TI  - SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination
KW  - Staphylococcus
KW  - bacteriophage
KW  - Myoviridae
KW  - endolysin
KW  - SH3b domain
KW  - NMR
UR  - http://events.embo.org/12-virus-microbe/
N2  - Staphylococcus aureus is a major causative agent of human and animal diseases. The increasing number of pathogenic strains resistant to antimicrobial drugs is a serious public health problem that can be solved by applications of phage therapy as a suitable alternative to antibiotics treatment. Currently the research focus on the phage encoded antimicrobial proteins applicable to combat bacterial infections, such as phage endolysins that digest the cell wall of bacteria. In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of SPO1-like viruses from family Myoviridae. Preliminary results of the NMR analysis confirmed the presence of the well defined structure of the studied construct, and provided the sequential assignment of all resonance frequencies along the peptide backbone allowing us to assess the presence of the secondary structure motifs.
ER  -

BENEŠÍK, Martin, Jiří NOVÁČEK, Lubomír JANDA, Radka DOPITOVÁ, Lukáš ŽÍDEK, Jiří DOŠKAŘ, Vladislava RŮŽIČKOVÁ, Marek MOŠA and Roman PANTŮČEK. SH3b cell wall binding domain of bacteriophage 812 endolysin: Cloning, expression and structure determination. In \textit{Viruses of Microbes. From exploration to applications in the -omics era. EMBO Conference Series, Brussels, Belgium}. 2012.

Detailed Information on Publication Record