DEJMKOVÁ, Eva, Marek ŠEBELA and Michaela WIMMEROVÁ. Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems. In XXIII.Biochemicky sjezd. 2012.
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Basic information
Original name Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems
Name in Czech Exprese hypotetického lektinu z pisum sativum-porovnání dvou eukaryotnich expresnich organismu
Authors DEJMKOVÁ, Eva (203 Czech Republic, belonging to the institution), Marek ŠEBELA (203 Czech Republic) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition XXIII.Biochemicky sjezd, 2012.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/12:00057853
Organization unit Faculty of Science
Keywords (in Czech) Lektin; Pisum sativum; Pichia pastoris; Saccharomyces cerevisiae
Keywords in English Lectin; Pisum sativum; Pichia Pastoris; Saccharomyces cerevisiae
Changed by Changed by: prof. RNDr. Michaela Wimmerová, Ph.D., učo 854. Changed: 22/1/2013 10:04.
Abstract
We have identified a new putative lectin from Pisum sativum using SDS-PAGE and MS analysis of highly purified pea amine oxidase where it was present as an impurity. This study was aimed at the production of this protein in a eucaryotic expression system and its structure-function characterization. As production of the native protein was found very low, we have compared two yeast expression systems - Pichia pastoris and Saccharomyces cerevisiae. Advantages/disadvantages of both approaches will be discussed in detail.
Abstract (in Czech)
Protein predikovaný jako lektin byl nalezen pomocí SDS-PAGE a MS analýzy při purifikaci aminooxidázy z hrachu, kde byl přítomen jako nečistota. Protože produkce nativního proteinu v prokaryotním organismu Escheriachia coli byla velmi nízká, byly vybrány dva systémy kvasinkových expresních organismů - Pichia pastoris a Saccharomyces cerevisiae. Studie je zaměřena na porovnání exprese cílového proteinu ve dvou eukaryotnich expresních systémech, další práce bude pokračovat funkčními studiemi a určení struktury proteinu.
Links
CZ.1.05/1.1.00/02.0068, interní kód MUName: CEITEC - středoevropský technologický institut (Acronym: CEITEC)
Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence
GA522/08/0555, research and development projectName: Strukturně-funkční charakterizace oxidoreduktas působících na dusíkaté regulační látky v rostlinách
Investor: Czech Science Foundation, Structure-functional characterization of oxidoreductases acting on nitrogenous regulatory compounds in plants
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