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@article{1075190,
author = {Flasarová, Magdalena and Pospíšilová, Petra and Mikalová, Lenka and Vališová, Zuzana and Dastychová, Eliška and Strnadel, Radim and Kuklová, Ivana and Woznicová, Vladana and Zákoucká, Hana and Šmajs, David},
article_number = {6},
doi = {http://dx.doi.org/10.2340/00015555-1335},
keywords = {Treponema pallidum; syphilis; molecular typing; macrolide resistance; nested PCR},
language = {eng},
issn = {0001-5555},
journal = {Acta Dermato-Venereologica},
title = {Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain},
volume = {92},
year = {2012}
}
TY - JOUR
ID - 1075190
AU - Flasarová, Magdalena - Pospíšilová, Petra - Mikalová, Lenka - Vališová, Zuzana - Dastychová, Eliška - Strnadel, Radim - Kuklová, Ivana - Woznicová, Vladana - Zákoucká, Hana - Šmajs, David
PY - 2012
TI - Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
JF - Acta Dermato-Venereologica
VL - 92
IS - 6
SP - 669–674
EP - 669–674
SN - 00015555
KW - Treponema pallidum
KW - syphilis
KW - molecular typing
KW - macrolide resistance
KW - nested PCR
N2 - A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.
ER -
FLASAROVÁ, Magdalena, Petra POSPÍŠILOVÁ, Lenka MIKALOVÁ, Zuzana VALIŠOVÁ, Eliška DASTYCHOVÁ, Radim STRNADEL, Ivana KUKLOVÁ, Vladana WOZNICOVÁ, Hana ZÁKOUCKÁ and David ŠMAJS. Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain. \textit{Acta Dermato-Venereologica}. 2012, vol.~92, No~6, p.~669–674. ISSN~0001-5555. Available from: https://dx.doi.org/10.2340/00015555-1335.
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