Whole genome sequence of Treponema pallidum subspecies pallidum
strain Mexico A

a 2011

Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A

PĚTROŠOVÁ, Helena, Petra POSPÍŠILOVÁ, Marie ZOBANÍKOVÁ, Lenka MIKALOVÁ, David ŠMAJS et. al.

Basic information

Original name

Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A

Name in Czech

Kompletní genomová sekvence Treponema pallidum subsp. pallidum kmene Mexico A

Authors

PĚTROŠOVÁ, Helena, Petra POSPÍŠILOVÁ, Marie ZOBANÍKOVÁ, Lenka MIKALOVÁ and David ŠMAJS

Edition

XV. Setkání biochemiků a molekulárních biologů, 2011

Other information

Type of outcome

Konferenční abstrakt

Confidentiality degree

není předmětem státního či obchodního tajemství

ISBN

978-80-210-5594-0

Keywords (in Czech)

Treponema pallidum, Mexico A, syfilis, celogenomové sekvenování

Keywords in English

Treponema pallidum, Mexico A, syphilis, whole genome sequencing

Změněno: 29/11/2012 12:55, Mgr. Barbora Štaudová

Abstract

V originále

Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis and is known as stealth pathogen for its ability to avoid immune response. A major advancement in research of this enigmatic bacterium was determination of whole genome sequence of TPA strain Nichols. Up to this date another two sequences of TPA strains were revealed. In parallel, genomes of Treponema pallidum ssp. pertenue (TPE) strains have been sequenced. TPE is the causative agent of yaws. Although syphilis and yaws are diseases with distinct clinical manifestations, genomic differences between TPA and TPE strains are minimal (0.02%). Here we present whole genome sequence of TPA strain Mexico A, which in some areas combines sequences from both TPA and TPE strains. The Mexico A genome was sequenced by Illumina (Solexa), a new generation sequencing method, and by Sanger sequencing. The length of Mexico A genome was determined to 1 140 041 bp, being the largest TPA genome. Whole genome sequence of TPA strain Mexico A was compared with genomes of TPA (Nichols, SS14 and Chicago) and TPE (Samoa D, Gauthier, CDC-2) strains. There are two mosaic genes in the Mexico A genome – TP0326 and TP0488. TP0326 encodes outer membrane protein, which is essential for membrane biogenesis, and TP0488 encodes methyl-accepting chemotaxis protein. Both proteins are significantly expressed during experimental rabbit infection and belong to small group of treponemal outer membrane proteins. Mosaic character of these genes is probably caused by accepting part of DNA from TPE strain during syphilis and yaws co-infection. It is the first documented evidence of horizontal gene transfer in Treponema pallidum species.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

Citovat

PĚTROŠOVÁ, Helena, Petra POSPÍŠILOVÁ, Marie ZOBANÍKOVÁ, Lenka MIKALOVÁ and David ŠMAJS. Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A. In XV. Setkání biochemiků a molekulárních biologů. 2011. ISBN 978-80-210-5594-0.

@proceedings{1075288,
   author = {Pětrošová, Helena and Pospíšilová, Petra and Zobaníková, Marie and Mikalová, Lenka and Šmajs, David},
   booktitle = {XV. Setkání biochemiků a molekulárních biologů},
   keywords = {Treponema pallidum, Mexico A, syphilis, whole genome sequencing},
   isbn = {978-80-210-5594-0},
   title = {Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A},
   year = {2011}
}

TY  - CONF
ID  - 1075288
AU  - Pětrošová, Helena - Pospíšilová, Petra - Zobaníková, Marie - Mikalová, Lenka - Šmajs, David
PY  - 2011
TI  - Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A
SN  - 9788021055940
KW  - Treponema pallidum, Mexico A, syphilis, whole genome sequencing
N2  - Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis and is known as stealth pathogen for its ability to avoid immune response. A major advancement in research of this enigmatic bacterium was determination of whole genome sequence of TPA strain Nichols. Up to this date another two sequences of TPA strains were revealed. In parallel, genomes of Treponema pallidum ssp. pertenue (TPE) strains have been sequenced. TPE is the causative agent of yaws. Although syphilis and yaws are diseases with distinct clinical manifestations, genomic differences between TPA and TPE strains are minimal (0.02%). Here we present whole genome sequence of TPA strain Mexico A, which in some areas combines sequences from both TPA and TPE strains. The Mexico A genome was sequenced by Illumina (Solexa), a new generation sequencing method, and by Sanger sequencing. The length of Mexico A genome was determined to 1 140 041 bp, being the largest TPA genome. Whole genome sequence of TPA strain Mexico A was compared with genomes of TPA (Nichols, SS14 and Chicago) and TPE (Samoa D, Gauthier, CDC-2) strains. There are two mosaic genes in the Mexico A genome – TP0326 and TP0488. TP0326 encodes outer membrane protein, which is essential for membrane biogenesis, and TP0488 encodes methyl-accepting chemotaxis protein. Both proteins are significantly expressed during experimental rabbit infection and belong to small group of treponemal outer membrane proteins. Mosaic character of these genes is probably caused by accepting part of DNA from TPE strain during syphilis and yaws co-infection. It is the first documented evidence of horizontal gene transfer in Treponema pallidum species.
ER  -

PĚTROŠOVÁ, Helena, Petra POSPÍŠILOVÁ, Marie ZOBANÍKOVÁ, Lenka MIKALOVÁ and David ŠMAJS. Whole genome sequence of Treponema pallidum subspecies pallidum strain Mexico A. In \textit{XV. Setkání biochemiků a molekulárních biologů}. 2011. ISBN~978-80-210-5594-0.

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