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@inproceedings{1075318,
author = {Michalcová, Lenka and Dopitová, Radka and Hejátko, Jan and Válková, Martina and Preisler, Jan},
address = {Brno},
booktitle = {CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis},
edition = {1. vyd.},
keywords = {CE-LIF; GF; fusion protein AHP5-GFP},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-904959-1-3},
pages = {186-189},
publisher = {9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012},
title = {Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF},
url = {http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf},
year = {2012}
}
TY - JOUR
ID - 1075318
AU - Michalcová, Lenka - Dopitová, Radka - Hejátko, Jan - Válková, Martina - Preisler, Jan
PY - 2012
TI - Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF
PB - 9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012
CY - Brno
SN - 9788090495913
KW - CE-LIF
KW - GF
KW - fusion protein AHP5-GFP
UR - http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf
L2 - http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf
N2 - We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP.
ER -
MICHALCOVÁ, Lenka, Radka DOPITOVÁ, Jan HEJÁTKO, Martina VÁLKOVÁ a Jan PREISLER. Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF. In \textit{CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis}. 1. vyd. Brno: 9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012, 2012, s.~186-189. ISBN~978-80-904959-1-3.
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