PALACKÝ, Jan, Michaela VORLÍČKOVÁ, Iva KEJNOVSKÁ and Peter MOJZEŠ. Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study. Nucleic Acids Research. Oxford: Oxford University Press, 2013, vol. 41, No 2, p. 1005-1016. ISSN 0305-1048. doi:10.1093/nar/gks1135.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study
Authors PALACKÝ, Jan (203 Czech Republic), Michaela VORLÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Iva KEJNOVSKÁ (203 Czech Republic, belonging to the institution) and Peter MOJZEŠ (203 Czech Republic).
Edition Nucleic Acids Research, Oxford, Oxford University Press, 2013, 0305-1048.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 8.808
RIV identification code RIV/00216224:14740/13:00067549
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/nar/gks1135
UT WoS 000314121100034
Keywords in English NTRAMOLECULAR G-QUADRUPLEX; K+ SOLUTION; B-DNA; MELTING TRANSITIONS; POTASSIUM SOLUTION; POLARIZED RAMAN; NUCLEIC-ACIDS; SPECTROSCOPY; SEQUENCE; TETRAPLEX
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 31. 1. 2014 10:06.
Abstract
DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G3(TTAG3)3 in the presence of K+and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G3(TTAG3)3 at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G3(TTAG3)3 concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. The transition is synergically supported by increased K+ concentration. However, even for extremely high G3(TTAG3)3 and K+concentrations, an intramolecular antiparallel quadruplex is spontaneously formed from desalted non-quadruplex single-strand after addition of K+ ions. Thermal destabilization or long dwell time are necessary to induce interquadruplex transition. On the contrary, Na+-stabilized G3(TTAG3)3 retains its antiparallel folding regardless of the extremely high DNA and/or Na+concentrations, thermal destabilization or annealing.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
PrintDisplayed: 1. 7. 2022 05:15