| OVAERE, Margriet, Jiří ŠPONER, Judit ŠPONEROVÁ, Piet HERDEWIJN a Luc VAN MEERVELT. How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid: ribonucleic acid duplex. Nucleic Acids Research. Oxford, UK: Oxford Press, 2012, roč. 40, č. 15, s. 7573-7583. ISSN 0305-1048. doi:10.1093/nar/gks470. |
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@article{1076056,
author = {Ovaere, Margriet and Šponer, Jiří and Šponerová, Judit and Herdewijn, Piet and Van Meervelt, Luc},
article_location = {Oxford, UK},
article_number = {15},
doi = {http://dx.doi.org/10.1093/nar/gks470},
keywords = {BASIS-SET CONVERGENCE; DNA-BASE PAIRS; INTERACTION ENERGIES; CRYSTAL-STRUCTURE; MODIFIED SIRNAS; MODEL; RI-MP2; REFINEMENT; EFFICIENCY; COMPLEXES},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid: ribonucleic acid duplex},
url = {http://nar.oxfordjournals.org/content/early/2012/05/24/nar.gks470.short},
volume = {40},
year = {2012}
}
TY - JOUR
ID - 1076056
AU - Ovaere, Margriet - Šponer, Jiří - Šponerová, Judit - Herdewijn, Piet - Van Meervelt, Luc
PY - 2012
TI - How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid: ribonucleic acid duplex
JF - Nucleic Acids Research
VL - 40
IS - 15
SP - 7573-7583
EP - 7573-7583
PB - Oxford Press
SN - 03051048
KW - BASIS-SET CONVERGENCE
KW - DNA-BASE PAIRS
KW - INTERACTION ENERGIES
KW - CRYSTAL-STRUCTURE
KW - MODIFIED SIRNAS
KW - MODEL
KW - RI-MP2
KW - REFINEMENT
KW - EFFICIENCY
KW - COMPLEXES
UR - http://nar.oxfordjournals.org/content/early/2012/05/24/nar.gks470.short
L2 - http://nar.oxfordjournals.org/content/early/2012/05/24/nar.gks470.short
N2 - Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA: messenger RNA (mRNA) interactions, the crystal structure of the ANA: RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 angstrom resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this crystal structure confirms the potential of altritol-modified siRNAs. Moreover, in the ANA strands, a new type of intrastrand interactions was found between the O2' hydroxyl group of one residue and the sugar ring O4' atom of the next residue. These interactions were further investigated by quantum chemical methods. Besides hydration effects, these intrastrand hydrogen bonds may also contribute to the stability of ANA: RNA duplexes.
ER -
OVAERE, Margriet, Jiří ŠPONER, Judit ŠPONEROVÁ, Piet HERDEWIJN a Luc VAN MEERVELT. How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid: ribonucleic acid duplex. \textit{Nucleic Acids Research}. Oxford, UK: Oxford Press, 2012, roč.~40, č.~15, s.~7573-7583. ISSN~0305-1048. doi:10.1093/nar/gks470.
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