DUŠKOVÁ, Marta, Ondrej ŠEDO, Kateřina KŠICOVÁ, Zbyněk ZDRÁHAL and Renata KARPÍŠKOVÁ. Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS. International Journal of Food Microbiology. Amserdam: Elsevier BV, 2012, vol. 159, No 2, p. 107-114. ISSN 0168-1605. doi:10.1016/j.ijfoodmicro.2012.07.029.
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Basic information
Original name Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS
Authors DUŠKOVÁ, Marta (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Kateřina KŠICOVÁ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Renata KARPÍŠKOVÁ (203 Czech Republic, guarantor).
Edition International Journal of Food Microbiology, Amserdam, Elsevier BV, 2012, 0168-1605.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.425
RIV identification code RIV/00216224:14740/12:00062215
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.ijfoodmicro.2012.07.029
UT WoS 000310768400007
Keywords in English Bacterial identification; Species-specific PCR; Restriction analysis of the 16S-rRNA gene; MALDI-TOF MS profiling; Dairy products; Meat products
Tags ok, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Ondrej Šedo, Ph.D., učo 13589. Changed: 31. 3. 2014 15:22.
Abstract
Lactobacilli are bacteria with important implications in food and feed fermentation. Detailed knowledge of the lactobacilli composition is of high relevance to food and health control, various industrial or biotechnological applications, etc., but accurate identification of the Lactobacillus species is not an easy task In this study, three methods, i.e. polymerase chain reaction (PCR), amplified 165 rDNA restriction analysis with restrictase MseI (16S-ARDRA), and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were evaluated for their capabilities to identify Lactobacillus species. After comparison of the three methods on a set of 19 type/reference Lactobacillus strains, 148 strains isolated from dairy and meat products were characterized by PCR and MALDI-TOF MS. The strains were differentiated into nine and ten lactobacilli species by PCR and MALDI-TOF MS, respectively, with nine of these species being congruent The success rates of species level assignment were 77% for PCR and 93% for MALDI-TOF MS that proved superior in the species identification. However, to differentiate between closely related Lactobacillus species, MALDI-TOF MS needs to be used in combination with genotypic techniques to achieve a more reliable identification.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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