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@article{1076377, author = {Dušková, Marta and Šedo, Ondrej and Kšicová, Kateřina and Zdráhal, Zbyněk and Karpíšková, Renata}, article_location = {Amserdam}, article_number = {2}, doi = {http://dx.doi.org/10.1016/j.ijfoodmicro.2012.07.029}, keywords = {Bacterial identification; Species-specific PCR; Restriction analysis of the 16S-rRNA gene; MALDI-TOF MS profiling; Dairy products; Meat products}, language = {eng}, issn = {0168-1605}, journal = {International Journal of Food Microbiology}, title = {Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS}, url = {http://www.sciencedirect.com/science/article/pii/S0168160512004254}, volume = {159}, year = {2012} }
TY - JOUR ID - 1076377 AU - Dušková, Marta - Šedo, Ondrej - Kšicová, Kateřina - Zdráhal, Zbyněk - Karpíšková, Renata PY - 2012 TI - Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS JF - International Journal of Food Microbiology VL - 159 IS - 2 SP - 107-114 EP - 107-114 PB - Elsevier BV SN - 01681605 KW - Bacterial identification KW - Species-specific PCR KW - Restriction analysis of the 16S-rRNA gene KW - MALDI-TOF MS profiling KW - Dairy products KW - Meat products UR - http://www.sciencedirect.com/science/article/pii/S0168160512004254 L2 - http://www.sciencedirect.com/science/article/pii/S0168160512004254 N2 - Lactobacilli are bacteria with important implications in food and feed fermentation. Detailed knowledge of the lactobacilli composition is of high relevance to food and health control, various industrial or biotechnological applications, etc., but accurate identification of the Lactobacillus species is not an easy task In this study, three methods, i.e. polymerase chain reaction (PCR), amplified 165 rDNA restriction analysis with restrictase MseI (16S-ARDRA), and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were evaluated for their capabilities to identify Lactobacillus species. After comparison of the three methods on a set of 19 type/reference Lactobacillus strains, 148 strains isolated from dairy and meat products were characterized by PCR and MALDI-TOF MS. The strains were differentiated into nine and ten lactobacilli species by PCR and MALDI-TOF MS, respectively, with nine of these species being congruent The success rates of species level assignment were 77% for PCR and 93% for MALDI-TOF MS that proved superior in the species identification. However, to differentiate between closely related Lactobacillus species, MALDI-TOF MS needs to be used in combination with genotypic techniques to achieve a more reliable identification. ER -
DUŠKOVÁ, Marta, Ondrej ŠEDO, Kateřina KŠICOVÁ, Zbyněk ZDRÁHAL and Renata KARPÍŠKOVÁ. Identification of lactobacilli isolated from food by genotypic methods and MALDI-TOF MS. \textit{International Journal of Food Microbiology}. Amserdam: Elsevier BV, vol.~159, No~2, p.~107-114. ISSN~0168-1605. doi:10.1016/j.ijfoodmicro.2012.07.029. 2012.
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