High resolution structure of Aleuria aurantia lectin mutant reveals the principal of increased affinity

HOUSER, Josef, Patrick ROMANO and Michaela WIMMEROVÁ. High resolution structure of Aleuria aurantia lectin mutant reveals the principal of increased affinity. In Fourth joint BER II and BESSY II user meeting. 2012.

Basic information

• Original name: High resolution structure of Aleuria aurantia lectin mutant reveals the principal of increased affinity
• Authors: HOUSER, Josef (203 Czech Republic, belonging to the institution), Patrick ROMANO (840 United States of America) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Fourth joint BER II and BESSY II user meeting, 2012.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14740/12:00057948
• Organization unit: Central European Institute of Technology
• Keywords in English: AAL; lectin; X-ray structure

Abstract

Mutant of Aleuria aurantia lectin with increased affinity towards 1-6 bound fucose was prepared. We prepared protein crystals and collected high quality data. Solved structure revealed the principal of the binding, shading the light upon variability of lectin binding sites.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GA303/09/1168, research and development project: Name: Lektiny z lidských patogenů - struktura, funkce, inženýrství

Investor: Czech Science Foundation, Lectins from human pathogens - structure, function, engineering

• GD301/09/H004, research and development project: Name: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny

Investor: Czech Science Foundation

• ME08008, research and development project: Name: Návrh antibakteriálních a antivirových léků na bázi cukrů a glykomimetik

Investor: Ministry of Education, Youth and Sports of the CR, Design of Carbohydrates and Glycomimetics as Antibacterial and Antiviral Drugs, Research and Development Programme KONTAKT (ME)

• 286154, interní kód MU: Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)

Investor: European Union, Capacities