Determination of oxidative stress and activities of antioxidant
enzymes in guinea pigs treated with haloperidol

J 2013

Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ et. al.

Basic information

Original name

Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol

Authors

GUMULEC, Jaromír (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Marián HLAVNA (703 Slovakia, belonging to the institution), Tibor STRAČINA (703 Slovakia, belonging to the institution), Markéta SZTALMACHOVÁ (203 Czech Republic, belonging to the institution), Veronika TANHÄUSEROVÁ (203 Czech Republic, belonging to the institution), Lukáš PÁCAL (203 Czech Republic, belonging to the institution), Branislav RUTTKAY-NEDECKÝ (203 Czech Republic), Jiří SOCHOR (203 Czech Republic), Ondřej ZÍTKA (203 Czech Republic), Petr BABULA (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), René KIZEK (203 Czech Republic), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Experimental and Therapeutic Medicine, Athens, Spandidos Publications Ltd. 2013, 1792-0981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.941

RIV identification code

RIV/00216224:14110/13:00067578

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3892/etm.2012.822

UT WoS

000313764200021

Keywords in English

haloperidol; guinea pig; oxidative stress; superoxide dismutase; glutathione reductase; glutathione-S-transferase

Abstract

V originále

Guinea pigs (Cavia porcellus) were treated with haloperidol (HP), and free radical (FR) and ferric reducing antioxidant power (FRAP) assays were used to determine oxidative stress levels. Furthermore, the superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST) activity levels were detected and glucose levels and the reduced and oxidized glutathione (GSH/GSSG) ratio were measured in HP-treated and untreated guinea pigs. The present study demonstrated that the administration of HP causes significant oxidative stress in guinea pigs (P=0,022). In animals treated with HP, the activity of GST was significantly increased compared with a placebo (P=0,007). The elevation of SOD and GR activity levels and increase in the levels of glutathione (GSH) in HP-treated animals were not statistically significant. In the HP-untreated animals, a significant positive correlation was observed between oxidative stress detected by the FR method and GST (r=0,88, P=0,008) and SOD (r=0,86, P=0,01) activity levels, respectively. A significant negative correlation between the levels of plasma glucose and oxidative stress detected by the FRAP method was observed (r=-0,78, P=0,04). Notably, no significant correlations were observed in the treated animals. In the HP-treated group, two subgroups of animals were identified according to their responses to oxidative stress. The group with higher levels of plasma HP had higher enzyme activity and reactive oxygen species production compared with the group with lower plasma levels of HP. The greatest difference in activity (U/microl) between the two groups of animals was for GR.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

MUNI/A/0846/2011, interní kód MU

Name: Studium mechanismů vzniku některých kardiovaskulárních onemocnění (Acronym: SMEKAON)

Investor: Masaryk University, Category A

Files attached

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Citovat

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Lukáš PÁCAL, Branislav RUTTKAY-NEDECKÝ, Jiří SOCHOR, Ondřej ZÍTKA, Petr BABULA, Vojtěch ADAM, René KIZEK, Marie NOVÁKOVÁ and Michal MASAŘÍK. Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol. Experimental and Therapeutic Medicine. Athens: Spandidos Publications Ltd., 2013, vol. 5, No 2, p. 479-484. ISSN 1792-0981. Available from: https://dx.doi.org/10.3892/etm.2012.822.

@article{1079005,
   author = {Gumulec, Jaromír and Raudenská, Martina and Hlavna, Marián and Stračina, Tibor and Sztalmachová, Markéta and Tanhäuserová, Veronika and Pácal, Lukáš and RuttkayandNedecký, Branislav and Sochor, Jiří and Zítka, Ondřej and Babula, Petr and Adam, Vojtěch and Kizek, René and Nováková, Marie and Masařík, Michal},
   article_location = {Athens},
   article_number = {2},
   doi = {http://dx.doi.org/10.3892/etm.2012.822},
   keywords = {haloperidol; guinea pig; oxidative stress; superoxide dismutase; glutathione reductase; glutathione-S-transferase},
   language = {eng},
   issn = {1792-0981},
   journal = {Experimental and Therapeutic Medicine},
   title = {Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol},
   volume = {5},
   year = {2013}
}

TY  - JOUR
ID  - 1079005
AU  - Gumulec, Jaromír - Raudenská, Martina - Hlavna, Marián - Stračina, Tibor - Sztalmachová, Markéta - Tanhäuserová, Veronika - Pácal, Lukáš - Ruttkay-Nedecký, Branislav - Sochor, Jiří - Zítka, Ondřej - Babula, Petr - Adam, Vojtěch - Kizek, René - Nováková, Marie - Masařík, Michal
PY  - 2013
TI  - Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol
JF  - Experimental and Therapeutic Medicine
VL  - 5
IS  - 2
SP  - 479-484
EP  - 479-484
PB  - Spandidos Publications Ltd.
SN  - 17920981
KW  - haloperidol
KW  - guinea pig
KW  - oxidative stress
KW  - superoxide dismutase
KW  - glutathione reductase
KW  - glutathione-S-transferase
N2  - Guinea pigs (Cavia porcellus) were treated with haloperidol (HP), and free radical (FR) and ferric reducing antioxidant power (FRAP) assays were used to determine oxidative stress levels. Furthermore, the superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST) activity levels were detected and glucose levels and the reduced and oxidized glutathione (GSH/GSSG) ratio were measured in HP-treated and untreated guinea pigs. The present study demonstrated that the administration of HP causes significant oxidative stress in guinea pigs (P=0,022). In animals treated with HP, the activity of GST was significantly increased compared with a placebo (P=0,007). The elevation of SOD and GR activity levels and increase in the levels of glutathione (GSH) in HP-treated animals were not statistically significant. In the HP-untreated animals, a significant positive correlation was observed between oxidative stress detected by the FR method and GST (r=0,88, P=0,008) and SOD (r=0,86, P=0,01) activity levels, respectively. A significant negative correlation between the levels of plasma glucose and oxidative stress detected by the FRAP method was observed (r=-0,78, P=0,04). Notably, no significant correlations were observed in the treated animals. In the HP-treated group, two subgroups of animals were identified according to their responses to oxidative stress. The group with higher levels of plasma HP had higher enzyme activity and reactive oxygen species production compared with the group with lower plasma levels of HP. The greatest difference in activity (U/microl) between the two groups of animals was for GR.
ER  -

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Lukáš PÁCAL, Branislav RUTTKAY-NEDECKÝ, Jiří SOCHOR, Ondřej ZÍTKA, Petr BABULA, Vojtěch ADAM, René KIZEK, Marie NOVÁKOVÁ and Michal MASAŘÍK. Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol. \textit{Experimental and Therapeutic Medicine}. Athens: Spandidos Publications Ltd., 2013, vol.~5, No~2, p.~479-484. ISSN~1792-0981. Available from: https://dx.doi.org/10.3892/etm.2012.822.

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