PAKOSTOVÁ, Eva, Martin MANDL, Blanka OMESOVÁ POKORNÁ, Eva DIVÍŠKOVÁ a Antonín LOJEK. Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur. Geomicrobiology Journal. PHILADELPHIA, USA: TAYLOR & FRANCIS INC, roč. 30, č. 1, s. 1-7. ISSN 0149-0451. doi:10.1080/01490451.2011.636790. 2013. |
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@article{1079022, author = {Pakostová, Eva and Mandl, Martin and Omesová Pokorná, Blanka and Divíšková, Eva and Lojek, Antonín}, article_location = {PHILADELPHIA, USA}, article_number = {1}, doi = {http://dx.doi.org/10.1080/01490451.2011.636790}, keywords = {Acidithiobacillus; acidophiles; ATP assay; iron-oxidizing bacteria; sulfur-oxidizing bacteria}, language = {eng}, issn = {0149-0451}, journal = {Geomicrobiology Journal}, title = {Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur}, volume = {30}, year = {2013} }
TY - JOUR ID - 1079022 AU - Pakostová, Eva - Mandl, Martin - Omesová Pokorná, Blanka - Divíšková, Eva - Lojek, Antonín PY - 2013 TI - Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur JF - Geomicrobiology Journal VL - 30 IS - 1 SP - 1-7 EP - 1-7 PB - TAYLOR & FRANCIS INC SN - 01490451 KW - Acidithiobacillus KW - acidophiles KW - ATP assay KW - iron-oxidizing bacteria KW - sulfur-oxidizing bacteria N2 - Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation of ferrous iron and elemental sulfur. Inhibitory effects of inorganic substrates and products on luciferase were eliminated. Extracellular ATP levels were negligible. The ATP content of sulfur-grown cells decreased anomalously due to a culture pH increase at the stationary phase. Although the rates of growth and sulfur oxidation reached the original levels, the ATP content of the culture remained constant because of gradual decrease in the cellular ATP. The maximum ATP levels in A. ferrooxidans grown with Fe(II) and S(0) were 1.16 and 0.33 amol per cell, respectively. The results defined conditions under which biomass growth could be monitored by the ATP assay to study biogeochemical activities of acidophilic iron- and sulfur-oxidizing bacteria. ER -
PAKOSTOVÁ, Eva, Martin MANDL, Blanka OMESOVÁ POKORNÁ, Eva DIVÍŠKOVÁ a Antonín LOJEK. Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur. \textit{Geomicrobiology Journal}. PHILADELPHIA, USA: TAYLOR \&{} FRANCIS INC, roč.~30, č.~1, s.~1-7. ISSN~0149-0451. doi:10.1080/01490451.2011.636790. 2013.
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