Piezoelectric and surface plasmon resonance biosensors for
Bacillus atrophaeus spores

J 2013

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL and Petr SKLÁDAL

Basic information

Original name

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

Name in Czech

Piezoelektrické a SPR biosensory pro stanoveni spor Bacillus atrophaeus

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2013, 1452-3981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Serbia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.956

RIV identification code

RIV/00216224:14310/13:00067588

Organization unit

Faculty of Science

UT WoS

000316562800008

Keywords (in Czech)

biosensor; spory; piezosensor

Keywords in English

Bacillus atrophaeus spore; Biosensor; Immunosensor; Quartz Crystal Microbalance; Surface Plasmon Resonance

Abstract

ORIG CZ

V originále

Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, food processing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbial biosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.

In Czech

Imunospecifické biosensory - imunosensory - pro detekci spor Bacillus atrophaeus, konstrukce na bázi piezosensorů a plasmonové resonance.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL and Petr SKLÁDAL. Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2013, vol. 8, No 1, p. 100-112. ISSN 1452-3981.

@article{1079670,
   author = {Farka, Zdeněk and Kovář, David and Přibyl, Jan and Skládal, Petr},
   article_location = {Belgrade, Serbia},
   article_number = {1},
   keywords = {Bacillus atrophaeus spore; Biosensor; Immunosensor; Quartz Crystal Microbalance; Surface Plasmon Resonance},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores},
   url = {http://www.electrochemsci.org/papers/vol8/80100100.pdf},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1079670
AU  - Farka, Zdeněk - Kovář, David - Přibyl, Jan - Skládal, Petr
PY  - 2013
TI  - Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores
JF  - International Journal of Electrochemical Science
VL  - 8
IS  - 1
SP  - 100-112
EP  - 100-112
PB  - ESG
SN  - 14523981
KW  - Bacillus atrophaeus spore
KW  - Biosensor
KW  - Immunosensor
KW  - Quartz Crystal Microbalance
KW  - Surface Plasmon Resonance
UR  - http://www.electrochemsci.org/papers/vol8/80100100.pdf
L2  - http://www.electrochemsci.org/papers/vol8/80100100.pdf
N2  - Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, food processing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbial biosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.
ER  -

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL and Petr SKLÁDAL. Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores. \textit{International Journal of Electrochemical Science}. Belgrade, Serbia: ESG, 2013, vol.~8, No~1, p.~100-112. ISSN~1452-3981.

Detailed Information on Publication Record