Gene expression profiling of acute graft-vs-host disease after
hematopoietic stem cell transplantation

J 2012

Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation

VERNER, Jan, Jitka KABÁTHOVÁ, Alexandra TOMANCOVÁ, Šárka PAVLOVÁ, Boris TICHÝ et. al.

Basic information

Original name

Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation

Authors

VERNER, Jan (203 Czech Republic, belonging to the institution), Jitka KABÁTHOVÁ (203 Czech Republic, belonging to the institution), Alexandra TOMANCOVÁ (203 Czech Republic, belonging to the institution), Šárka PAVLOVÁ (203 Czech Republic, belonging to the institution), Boris TICHÝ (203 Czech Republic, belonging to the institution), Marek MRÁZ (203 Czech Republic, belonging to the institution), Yvona BRYCHTOVÁ (203 Czech Republic, belonging to the institution), Marta KREJČÍ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Martin TRBUŠEK (203 Czech Republic, belonging to the institution), Jana VOLEJNÍKOVÁ (203 Czech Republic), Petr SEDLÁČEK (203 Czech Republic), Michael DOUBEK (203 Czech Republic, belonging to the institution), Jiří MAYER (203 Czech Republic, belonging to the institution) and Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Experimental Hematology, New York, Elsevier Science, 2012, 0301-472X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.907

RIV identification code

RIV/00216224:14740/12:00062583

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.exphem.2012.06.011

UT WoS

000310182400004

Keywords in English

MONONUCLEAR-CELLS; ACUTE GVHD; BLOOD; MICROARRAYS; BIOMARKERS; THERAPY

Abstract

V originále

Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups-favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8, GOS2, ANXA3, and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes. (C) 2012 ISEH - Society for Hematology and Stem Cells.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0045, research and development project

Name: Podpora profesního růstu a mezinárodní integrace výzkumných týmů v oblasti molekulární medicíny

MSM0021622430, plan (intention)

Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

NS9683, research and development project

Name: Změny genomu a proteomu u leukemických pacientů s reakcí štěpu proti hostiteli (GvHD)

Investor: Ministry of Health of the CR

Citovat

VERNER, Jan, Jitka KABÁTHOVÁ, Alexandra TOMANCOVÁ, Šárka PAVLOVÁ, Boris TICHÝ, Marek MRÁZ, Yvona BRYCHTOVÁ, Marta KREJČÍ, Zbyněk ZDRÁHAL, Martin TRBUŠEK, Jana VOLEJNÍKOVÁ, Petr SEDLÁČEK, Michael DOUBEK, Jiří MAYER and Šárka POSPÍŠILOVÁ. Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation. Experimental Hematology. New York: Elsevier Science, 2012, vol. 40, No 11, p. 899-905. ISSN 0301-472X. Available from: https://dx.doi.org/10.1016/j.exphem.2012.06.011.

@article{1080443,
   author = {Verner, Jan and Kabáthová, Jitka and Tomancová, Alexandra and Pavlová, Šárka and Tichý, Boris and Mráz, Marek and Brychtová, Yvona and Krejčí, Marta and Zdráhal, Zbyněk and Trbušek, Martin and Volejníková, Jana and Sedláček, Petr and Doubek, Michael and Mayer, Jiří and Pospíšilová, Šárka},
   article_location = {New York},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.exphem.2012.06.011},
   keywords = {MONONUCLEAR-CELLS; ACUTE GVHD; BLOOD; MICROARRAYS; BIOMARKERS; THERAPY},
   language = {eng},
   issn = {0301-472X},
   journal = {Experimental Hematology},
   title = {Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/22771791},
   volume = {40},
   year = {2012}
}

TY  - JOUR
ID  - 1080443
AU  - Verner, Jan - Kabáthová, Jitka - Tomancová, Alexandra - Pavlová, Šárka - Tichý, Boris - Mráz, Marek - Brychtová, Yvona - Krejčí, Marta - Zdráhal, Zbyněk - Trbušek, Martin - Volejníková, Jana - Sedláček, Petr - Doubek, Michael - Mayer, Jiří - Pospíšilová, Šárka
PY  - 2012
TI  - Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation
JF  - Experimental Hematology
VL  - 40
IS  - 11
SP  - 899-905
EP  - 899-905
PB  - Elsevier Science
SN  - 0301472X
KW  - MONONUCLEAR-CELLS
KW  - ACUTE GVHD
KW  - BLOOD
KW  - MICROARRAYS
KW  - BIOMARKERS
KW  - THERAPY
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22771791
L2  - http://www.ncbi.nlm.nih.gov/pubmed/22771791
N2  - Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups-favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8, GOS2, ANXA3, and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes. (C) 2012 ISEH - Society for Hematology and Stem Cells.
ER  -

VERNER, Jan, Jitka KABÁTHOVÁ, Alexandra TOMANCOVÁ, Šárka PAVLOVÁ, Boris TICHÝ, Marek MRÁZ, Yvona BRYCHTOVÁ, Marta KREJČÍ, Zbyněk ZDRÁHAL, Martin TRBUŠEK, Jana VOLEJNÍKOVÁ, Petr SEDLÁČEK, Michael DOUBEK, Jiří MAYER and Šárka POSPÍŠILOVÁ. Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation. \textit{Experimental Hematology}. New York: Elsevier Science, 2012, vol.~40, No~11, p.~899-905. ISSN~0301-472X. Available from: https://dx.doi.org/10.1016/j.exphem.2012.06.011.

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