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@article{1081533, author = {Kunová, Michaela and Matulka, Kamil and Eiselleová, Lívia and Salykin, Anton and Kubíková, Iva and Kyrylenko, Sergiy and Hampl, Aleš and Dvořák, Petr}, article_location = {DURHAM}, article_number = {4}, doi = {http://dx.doi.org/10.5966/sctm.2012-0081}, keywords = {Embryonic stem cells; Cell culture; Xenograft assay; Cell survival; Induced pluripotent stem cells}, language = {eng}, issn = {2157-6564}, journal = {STEM CELLS Translational Medicine}, title = {Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability}, volume = {2}, year = {2013} }
TY - JOUR ID - 1081533 AU - Kunová, Michaela - Matulka, Kamil - Eiselleová, Lívia - Salykin, Anton - Kubíková, Iva - Kyrylenko, Sergiy - Hampl, Aleš - Dvořák, Petr PY - 2013 TI - Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability JF - STEM CELLS Translational Medicine VL - 2 IS - 4 SP - 246-254 EP - 246-254 PB - ALPHAMED PRESS SN - 21576564 KW - Embryonic stem cells KW - Cell culture KW - Xenograft assay KW - Cell survival KW - Induced pluripotent stem cells N2 - The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies. ER -
KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Anton SALYKIN, Iva KUBÍKOVÁ, Sergiy KYRYLENKO, Aleš HAMPL and Petr DVOŘÁK. Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability. \textit{STEM CELLS Translational Medicine}. DURHAM: ALPHAMED PRESS, 2013, vol.~2, No~4, p.~246-254. ISSN~2157-6564. Available from: https://dx.doi.org/10.5966/sctm.2012-0081.
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