Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Anton SALYKIN, Iva KUBÍKOVÁ, Sergiy KYRYLENKO, Aleš HAMPL and Petr DVOŘÁK. Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability. STEM CELLS Translational Medicine. DURHAM: ALPHAMED PRESS, 2013, vol. 2, No 4, p. 246-254. ISSN 2157-6564. Available from: https://dx.doi.org/10.5966/sctm.2012-0081.

Basic information

• Original name: Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability
• Authors: KUNOVÁ, Michaela (203 Czech Republic, guarantor, belonging to the institution), Kamil MATULKA (203 Czech Republic, belonging to the institution), Lívia EISELLEOVÁ (703 Slovakia, belonging to the institution), Anton SALYKIN (643 Russian Federation, belonging to the institution), Iva KUBÍKOVÁ (203 Czech Republic, belonging to the institution), Sergiy KYRYLENKO (246 Finland, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Petr DVOŘÁK (203 Czech Republic, belonging to the institution).
• Edition: STEM CELLS Translational Medicine, DURHAM, ALPHAMED PRESS, 2013, 2157-6564.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.596
• RIV identification code: RIV/00216224:14110/13:00065980
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.5966/sctm.2012-0081
• UT WoS: 000317551800002
• Keywords in English: Embryonic stem cells; Cell culture; Xenograft assay; Cell survival; Induced pluripotent stem cells
• Tags: International impact, Reviewed

Abstract

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.

Links

• GBP302/12/G157, research and development project: Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

• LC06077, research and development project: Name: Centrum chemické genetiky

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622430, plan (intention): Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies