The role of sterol binding and surface charge in 
elicitin-induced resistance

a 2011

The role of sterol binding and surface charge in elicitin-induced resistance

LOCHMAN, Jan, Michal OBOŘIL, Veronika PLEŠKOVÁ, Kateřina DADÁKOVÁ, Nikola PTÁČKOVÁ et. al.

Basic information

Original name

The role of sterol binding and surface charge in elicitin-induced resistance

Name in Czech

Úloha vazby sterolu a povrchového náboje v biologické aktivitě elicitinů

Authors

LOCHMAN, Jan, Michal OBOŘIL, Veronika PLEŠKOVÁ, Kateřina DADÁKOVÁ, Nikola PTÁČKOVÁ, Zbyněk ZDRÁHAL and Tomáš KAŠPAROVSKÝ

Edition

10th conference of the EFPP “IPM 2.0" 2011

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Abstract

V originále

Elicitins are family of small proteins secreted by species of the pathogenic fungus Phytophthora inducing a defence reaction in plants. On the basis of their pI they are classified as either alpha elicitins or more necrotizing beta elicitins. Cryptogein is beta elicitin secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. It contains a hydrophobic cavity capable of binding sterols and fatty acids and highly conserved omega-loop. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind lipids and with influenced surface charge. The sterol binding and phospholipids transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However importance of omega-loop for interaction of the protein with the high affinity binding site on the plasma membrane was proved.

Links

CZ.1.07/2.4.00/31.0133, interní kód MU

Name: BiochemNet - Vytvoření sítě pro podporu spolupráce biomedicínských pracovišť a zvýšení uplatnitelnosti absolventů biochemických oborů v praxi

Investor: Ministry of Education, Youth and Sports of the CR, 2.4 Partnership and networks

GAP501/11/1003, research and development project

Name: Interakce mutantních forem cryptogeinu s membránovým vazebným místem: charakterizace pomocí biosensorů

Investor: Czech Science Foundation

Citovat

LOCHMAN, Jan, Michal OBOŘIL, Veronika PLEŠKOVÁ, Kateřina DADÁKOVÁ, Nikola PTÁČKOVÁ, Zbyněk ZDRÁHAL and Tomáš KAŠPAROVSKÝ. The role of sterol binding and surface charge in elicitin-induced resistance. In 10th conference of the EFPP “IPM 2.0". 2011.

@proceedings{1082093,
   author = {Lochman, Jan and Obořil, Michal and Plešková, Veronika and Dadáková, Kateřina and Ptáčková, Nikola and Zdráhal, Zbyněk and Kašparovský, Tomáš},
   booktitle = {10th conference of the EFPP “IPM 2.0"},
   language = {eng},
   title = {The role of sterol binding and surface charge in elicitin-induced resistance},
   year = {2011}
}

TY  - CONF
ID  - 1082093
AU  - Lochman, Jan - Obořil, Michal - Plešková, Veronika - Dadáková, Kateřina - Ptáčková, Nikola - Zdráhal, Zbyněk - Kašparovský, Tomáš
PY  - 2011
TI  - The role of sterol binding and surface charge in elicitin-induced resistance
N2  - Elicitins are family of small proteins secreted by species of the pathogenic fungus Phytophthora inducing a defence reaction in plants. On the basis of their pI they are classified as either alpha elicitins or more necrotizing beta elicitins. Cryptogein is beta elicitin secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. It contains a hydrophobic cavity capable of binding sterols and fatty acids and highly conserved omega-loop. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind lipids and with influenced surface charge. The sterol binding and phospholipids transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However importance of omega-loop for interaction of the protein with the high affinity binding site on the plasma membrane was proved.
ER  -

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