Detailed Information on Publication Record
2011
Development of Cryptosporidium muris in experimental conditions: preliminary study.
MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ and Andrea VALIGUROVÁBasic information
Original name
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Name in Czech
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Name (in English)
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Authors
MELICHEROVÁ, Janka (703 Slovakia, guarantor, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Martin KVÁČ (203 Czech Republic) and Andrea VALIGUROVÁ (703 Slovakia)
Edition
41. Jírovec Protozoological Days, Benecko v Krkonoších, May 9-13, 2011. 2011
Other information
Language
Czech
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/11:00058215
Organization unit
Faculty of Science
Keywords (in Czech)
cryptosporidia; life cycle; rodent
Keywords in English
cryptosporidia; life cycle; rodent
Změněno: 12/2/2014 15:02, Mgr. et Mgr. Jana Ilgová, Ph.D.
V originále
Cryptosporidia (Apicomplexa) have been recently recognized as one of the most ubiquitous parasites infecting gastrointestinal and respiratory tract of vertebrates. Localization of cryptosporidian developmental stages in the host epithelium has been the subject of extensive speculation for many years. Our published data support the term epicellular to reflect the cryptosporidian localization, rather than the term intracellular extracytoplasmic, used throughout the literature. Due to its peculiar localization, Cryptosporidium can derive nutrition while minimizing immunologic recognition and there is currently no effective treatment for cryptosporidiosis. In presented study, the life cycle of Cryptosporidium muris parasitizing gastric glands of experimentally infected laboratory rodents, Mastomys natalensis and BALB/c mice, has been analysed in detail. The main aim was to map the development of C. muris in hosts exhibiting different response to the parasitization. We have focused on the endogenous development of C. muris, during which the parasite was attached to the host epithelium and its development included both asexual and sexual developmental stages. Animals were dissected and screened for the presence of parasite using combined morphological approach and nested PCR (SSU rRNA) at different time after inoculation with infective oocysts. The study has revealed possible difficulties in detecting C. muris developmental stages in gastric glands during the first days of parasitization.
In English
Cryptosporidia (Apicomplexa) have been recently recognized as one of the most ubiquitous parasites infecting gastrointestinal and respiratory tract of vertebrates. Localization of cryptosporidian developmental stages in the host epithelium has been the subject of extensive speculation for many years. Our published data support the term epicellular to reflect the cryptosporidian localization, rather than the term intracellular extracytoplasmic, used throughout the literature. Due to its peculiar localization, Cryptosporidium can derive nutrition while minimizing immunologic recognition and there is currently no effective treatment for cryptosporidiosis. In presented study, the life cycle of Cryptosporidium muris parasitizing gastric glands of experimentally infected laboratory rodents, Mastomys natalensis and BALB/c mice, has been analysed in detail. The main aim was to map the development of C. muris in hosts exhibiting different response to the parasitization. We have focused on the endogenous development of C. muris, during which the parasite was attached to the host epithelium and its development included both asexual and sexual developmental stages. Animals were dissected and screened for the presence of parasite using combined morphological approach and nested PCR (SSU rRNA) at different time after inoculation with infective oocysts. The study has revealed possible difficulties in detecting C. muris developmental stages in gastric glands during the first days of parasitization.
Links
| GPP506/10/P372, research and development project |
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